摘要
目的 检测朊蛋白在口腔正常黏膜、白斑、鳞状细胞癌（oral squamous cell carcinomas,OSCC）以及其各亚组之间的表达及其临床意义.方法 应用免疫组化SP法检测OSCC（84例）、白斑（26例）及正常黏膜组织（20例）中朊蛋白的表达情况,并分析其在性别、种族、组织病理分期、临床分期等临床参数的相关性.结果 朊蛋白在正常黏膜、白斑及OSCC组织中的阳性表达率分别为15％（3/20）、42％ （11/26）和95％（80/84）;朊蛋白在白斑及OSCC中的表达差异有统计学意义（P＜0.05）,阳性率随分化程度下降而升高（P＜0.05）.朊蛋白在肿瘤TNM分期及性别、种族中的表达差异无统计学意义（P＞0.05）.在TNM分期中,I、Ⅱ期朊蛋白表达强度随分化下降而明显升高（P＜0.05）,但与Ⅲ、Ⅳ期之间的差异无统计学意义（P＞0.05）;而在I＋Ⅱ和Ⅲ＋Ⅳ期的总体表达差异中有统计学意义（P＜0.05）.结论 朊蛋白在OSCC中的高表达及在白斑、OSCC中的渐进性高表达与OSCC的发生、病理分期、及临床TNM分期密切相关,可为OSCC的早期诊断和靶向治疗提供依据.
Objective To examine Prion protein （PrP） expression and its clinical significance in oral mucosa,oral leukoplakia,oral squamous cell carcinoma（OSCC） and its subgroups.Methods Expression of PrP in OSCC,oral leukoplakia and mucosa specimen was detected by immunohistochemistry.The association between the expression and gender,TNM clinical stages,pathological grades was evaluated.Results The positive expression rate of PrP in normal,oral leukoplakia and OSCC tissues was 15 % （3/20）,42 % （11/26） and 95 % （80/84）,respectively.There was a significant difference between the expression of PrP in leukoplakia and in high,moderately and poorly differentiated OSCC （P 〈 0.05）.The positive expression rate was increased with the declining of pathological differentiation （P 〈 0.05）.There was no significant difference in PrP expression among lymph node metastasis and gender.PrP expression of stages I and Ⅱ was up-regulated with the decreased differentiation （P 〈 0.05）.There was no significant difference in PrP expression between stage Ⅲ and Ⅳ （P 〉 0.05）.Between stages Ⅰ ＋ Ⅱ and Ⅲ ＋ Ⅳ in the overa Ⅱ expression of PrP,there was a significant difference （P 〈 0.05）.Conclusions The high expression of PrP in OSCC and the progressive expression from leukoplakia to OSCC was closely related to the carcinogenesis of OSCC,pathologic stage and clinical TNM stage.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2013年第12期752-754,共3页
Chinese Journal of Stomatology
基金
中国科学院病毒学国家重点实验室开放研究基金（2013IOV002）
关键词
口腔鳞状细胞癌
白斑
朊蛋白
Oral sprays
Oral leukoplakia
Prion protein
