摘要
系统研究了干法消化-分光光度法测定食品中铝含量的最佳反应条件,建立了快速测定食品中铝含量的方法。研究了槲皮素用量、铝用量、缓冲溶液浓度、缓冲溶液pH值及反应时间对络合物吸光度的影响以及生成络合物的稳定性,并对不同食品中铝含量进行了测定。在乙酸盐的缓冲介质中,铝离子和槲皮素生成黄色络合物,测定波长为425 nm。缓冲液浓度为0.02~0.10 mol/L;缓冲溶液的pH为4.5~6.0;反应时间为20 min。吸光度与铝含量呈现良好的线性关系,根据曲线方程测定了食品中铝含量,样品中铝含量从高到低的顺序为海蜇皮、油条和面包。相对标准差为1.64%~3.76%,回收率为94.90%~100.60%。此方法测定食品中的铝含量简单快速,灵敏度高,稳定性好。
The optimum reaction conditions for the determination of aluminum in food by dry digestion-spectrophotometry were studied systematically,and the method of rapid determination of aluminum in food was established.The effects of quercetin content,aluminum dosage,buffer solution concentration,pH value of buffer solution and reaction time on the absorbance of the complex and the stability of the complex were studied.The aluminum content in different food was determined by this method.In the acetate buffer medium,the aluminum ions and quercetin produced the yellow complex,whose determination wavelength was 425 nm.The optimum conditions for determining aluminum in foods were as follows:0.02~0.10 mol/L of buffer solution concentration,4.5~6.0 of buffer solution pH value,and 20 min of reaction time.There was a good linearity between the absorbance and aluminum concentration.According to the curve equation,aluminum content in food was determined.The aluminum content in the samples was jellyfish>deep-fried dough sticks>bread in the order from high to low.The relative standard deviation was 1.64%~3.76%,and the recovery rate was 94.90%~100.60%.This method is simple and rapid and has a high sensitivity and good stability for the determination of aluminum in foods.
作者
韩笑
李佳琳
张美瑄
曹贵玲
鄂旭
李颖畅
HanXiao;Li Jialin;Zhang Meixuan;Cao Guiling;E Xu;Li Yingchang(College of Food Science and Technology,Bohai University,Jinzhou 121013,China;National&Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,Jinzhou 121013,China)
出处
《包装与食品机械》
CAS
北大核心
2019年第6期68-72,共5页
Packaging and Food Machinery
基金
国家科技支撑计划(2016YFD0400805)
关键词
铝测定
槲皮素
食品
分光光度法
aluminum determination
quercetin
food
spectrophotometric method
