摘要
以黄皮果核为原料提取多糖,采用响应面法优化料液比、提取温度、提取时间3个因素的提取工艺,并对黄皮果核多糖清除DPPH与ABTS自由基能力进行了研究。结果表明:料液比、提取温度显著影响多糖提取率,提取温度的影响最大,而提取时间的影响最小;最佳提取工艺为料液比1:52 g/mL、提取温度93℃、提取时间6 h,此条件下多糖的提取率为75.86%;黄皮果核多糖对DPPH与ABTS自由基清除能力分别为0.51±0.03μg Vc/μg、0.57±0.02μg Vc/μg,说明黄皮果核多糖具有较好的抗氧化活性。
Wampee seeds were used as the raw material to extract polysaccharides,and the extraction process was optimized by response surface methodology in terms of solid-liquid ratio,extraction temperature and extraction time,and the scavenging ability of DPPH and ABTS free radicals of polysaccharides was also investigated.The results show that the solid-liquid ratio and extraction temperature significantly affect the polysaccharide yield,extraction temperature has biggest effect,while extraction time has smallest effect.The optimal extraction process was solid-liquid ratio of 1:52 g/mL,extraction temperature of 93℃and extraction time of 6 h,under which the polysaccharide yield was 75.86%.The ability of polysaccharides to scavenge DPPH and ABTS free radicals was 0.51±0.03μg Vc/μg,0.57±0.02μg Vc/μg,respectively,indicating that the extracted polysaccharides have relatively good antioxidant activity.
作者
刘永
余嘉敏
Liu Yong;Yu Jiamin(School of Food and Pharmaceutical Engineering,Zhaoqing University,Guangdong Zhaoqing 526061,China)
出处
《包装与食品机械》
CAS
北大核心
2019年第6期14-17,共4页
Packaging and Food Machinery
基金
肇庆市科技计划项目(2014F012)
关键词
多糖提取
黄皮果核
抗氧化性
polysaccharide extraction
wampee seeds
antioxidant activity
