摘要
从云南大理市弥渡县石夹泉热泉的55℃底泥中筛选到1株高温脂肪酶的高产菌株,进行显微形态及生理生化特征、16S rRNA基因序列分析,将其初步鉴定为不动杆菌属(Acinetobacter sp.)的一株菌,命名为Acinetobacter sp. Lip-55。对其生长条件及酶学性质进行了研究,结果表明,该菌株耐高温性较好,菌株在55℃仍能生长,菌株最适生长温度为37℃。所产脂肪酶最适酶活温度为55℃,最适反应pH值为5.0。该酶在25℃以下,能保持良好的稳定性,Zn^2+、Ca^2+、Mg^2+对该酶有一定的抑制作用,Mn^2+、K^+、Fe^2+、Cu^2+均对该酶活力起到促进作用,其中Fe2+的促进效果最为显著。
A strain Lip-55 producing thermostable lipase was isolated from the sediment of Shijiaquan hot spring. It was identified as Acinetobacter sp. According to its morphology, physiological and biochemical characteristics, and 16 S rRNA sequence analysis. The growth conditions and enzymatic properties were studied. The results showed that it could grow at 55 ℃ and the optimum growth temperature was 37 ℃. The lipase had maximum activity at 55 ℃ and t pH 5.0. Zn^2+, Ca^2+ and Mg^2+ had a certain inhibitory effect on the enzyme activity. Mn^2+, K^+, Fe^2+ and Cu^2+ promoted the activity, of which Fe2+ was the most obvious.
作者
陈贵元
刘林波
桑鹏
杨力权
CHEN Guiyuan;LIU Linbo;SANG Peng;YANG Liquan(Basic Medical College,Dali University,Dali 671000,China;Key Laboratory of Insect Biomedical Research and Development of Yunnan Province,Dali 671000,China;College of Agriculture and Biological Science,Dali University,Dali 671003,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2019年第24期52-57,共6页
Food and Fermentation Industries
基金
国家自然科学基金项目(31860252)
国家自然科学基金项目(31660015)
云南省自然科学基金高校联合面上项目(2017FH001-084)
云南省自然科学基金项目(2017FB024)
关键词
高温脂肪酶
筛选
鉴定
酶学性质
thermostable lipase
isolation
identification
lipase-properties
