摘要
目的:探究复方七芍降压片对自发性高血压大鼠(SHR)血压及血管重塑的作用机制。方法:SHR大鼠随机分为模型组、厄贝沙坦片组、复方七芍降压片组,每组10只,另设10只WKY大鼠为正常对照组,给药6周,无创血压仪测量各周大鼠血压变化;HE染色观察大鼠肠系膜动脉病理变化并测量管壁厚度、血管中膜厚度及相同位置的管腔直径,比值记为WT;免疫组化法检测肠系膜动脉中基质金属蛋白酶-2(MMP-2)及Ⅰ、Ⅲ型胶原蛋白(COⅠ、COⅢ)表达情况。结果:给药6周后,与正常对照组比较,模型组大鼠各周血压均明显升高(P<0.01),管壁厚度及WT值增高(P<0.01或P<0.05),肠系膜动脉中MMP-2及Ⅰ、Ⅲ型胶原蛋白表达均明显升高(P<0.01);与模型组比较,复方七芍降压片组大鼠各周血压均明显降低(P<0.01),管壁厚度及WT值降低(P<0.05),肠系膜动脉中MMP-2及Ⅰ、Ⅲ型胶原蛋白表达均明显降低(P<0.05),且病理变化得到改善。结论:复方七芍降压片具有降低SHR大鼠血压,改善血管重塑的作用,其机制可能与降低MMP-2表达及Ⅰ、Ⅲ型胶原蛋白沉积有关。
AIM:To explore the mechanism of compound Qishao Jiangya tablet on blood pressure and vascular remodeling in spontaneously hypertensive rats(SHR).METHODS:SHR rats were randomly divided into model group,irbesartan tablet group and compound Qishao Jiangya tablet group,10 rats in each group;Another 10 WKY rats were used as normal control group;All received administration for 6 weeks;noninvasive sphygmomanometer was used to measure the changes of blood pressure in rats;HE staining was used to observe the pathological changes of mesenteric artery in rats and to measure the thickness of mesenteric artery wall;The thickness of intima media and the diameter of lumen in the same position were recorded as WT;Detection of matrix metalloprotease-2(MMP-2)and typeⅠand type Ⅲ collagen(COⅠ,CO Ⅲ)protein expression in mesenteric artery was performed by immuno-histochemical method.RESULTS:After 6 weeks of administration,compared with the normal control group,the blood pressure of the model group was significantly higher than that of the control group(P<0.01),and the thickness of tube wall and WT value were increased(P<0.01 or P<0.05),the expression of MMP-2,I and Ⅲ collagen in mesenteric artery increased significantly(P<0.01);Compared with model group,the blood pressure of the rats in the compound Qishao Jiangya tablet group was significantly lower than that in the control group(P<0.01),The thickness of tube wall and WT value decreased(P<0.05),the expression of MMP-2 and typeⅠand type Ⅲ collagen in mesenteric artery was significantly decreased(P<0.05),and the pathological changes were improved.CONCLUSION:Compound Qishaojiang tablet can reduce blood pressure and improve vascular remodeling in SHR rats,the mechanism may be related to the decrease of MMP-2 expression and the deposition of typeⅠand type Ⅲ collagen.
作者
李弘
刘叶倩
龚姗
刘林
曾勇
蔡晓
王宇红
任卫琼
LI Hong;LIU Yeqian;GONG Shan;LIU Lin;ZENG Yong;CAI Xiao;WANG Yuhong;REN Weiqiong(The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,Hunan,China;Innovation Centre for Science and Technology,Changsha 410208,Hunan,China)
出处
《中国临床药理学与治疗学》
CAS
CSCD
2019年第12期1364-1370,共7页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
湖南省中医药管理局重点项目(201910)
湖南省科技厅项目(2018SK51203)
中医内科重大疾病防治及转化教育部重点实验室开放基金(ZYNK201703)
研究生校级创新课题(2018CX37)
国家自然科学基金(81473616)
