摘要
目的观察自体血液穴位注射疗法治疗寻常型痤疮的临床效果,并从免疫调节方面探讨其治疗寻常型痤疮的部分作用机制。方法选择30例符合纳入标准的寻常型痤疮患者为治疗组,20例健康志愿者为对照组。治疗组采用自体血注射曲池、血海和足三里穴位,2次/周,4周为1个疗程,治疗1个疗程后评估临床效果,对照组不给予任何干预措施。采用流式细胞仪检测30例寻常型痤疮患者经自体血液穴位注射疗法治疗前后外周血CD14+单核细胞TLR2的表达,并与对照组20例健康志愿者进行比较。结果干预后,治疗组皮肤出油、皮损程度、数量、颜色、肿痛及皮损总积分均较干预前降低(P<0.05)。治疗组干预前外周血CD14+单核细胞TLR2的表达显著高于对照组(P<0.05);干预后,治疗组外周血CD14+单核细胞TLR2的表达显著低于干预前(P<0.05);治疗组干预后外周血CD14+单核细胞TLR2的表达与对照组无显著差异(P>0.05)。自血疗法治疗寻常型痤疮的总有效率达83.3%。结论自血疗法能够有效改善寻常型痤疮患者的皮损状态,其作用机制可能与降低寻常型痤疮患者外周血CD14+单核细胞TLR2的表达有关。
Objective To observe the clinical efficacy of self blood acupoint injection therapy in the treatment of acne vulgaris,and to explore its partial mechanism of action in the treatment of acne vulgaris from the aspect of immunoregulation.Methods Thirty acne vulgaris patients who met the inclusion criteria were selected as treatment group,20 healthy volunteers were selected as control group.The treatment group was treated with self blood injection at Quchi,Xuehai and Zusanli acupoints twice a week for 4 weeks,after one course of treatment,the clinical efficacy was evaluated.No intervention was given in the control group.The expression of TLR2 on CD14+monocyte in peripheral blood of 30 patients with acne vulgaris before and after the treatment of self blood acupoint injection therapy was detected by flow cytometry,and compared with that of 20 healthy volunteers in the control group.Results After intervention,the scores of skin oil,degree,quantity,color,swelling and pain of skin lesion and total score of skin lesion in the treatment group were lower than those before intervention(P<0.05).The expression of TLR2 on CD14+monocyte in peripheral blood in the treatment group before intervention was significantly higher than that in the control group(P<0.05);after intervention,the expression of TLR2 on CD14+monocyte in peripheral blood in the treatment group was significantly lower than that before intervention(P<0.05);there was no significant difference in the expression of TLR2 on CD14+monocyte between the treatment group after intervention and the control group(P>0.05).The total effective rate of autohemotherapy for acne vulgaris was 83.3%.Conclusion Autohemotherapy can effectively improve the skin condition of acne vulgaris patients,and its mechanism may be related to the decrease of TLR2 expression on CD14+monocyte in peripheral blood of patients with acne vulgaris.
作者
颜雪珍
屈强强
黄丽萍
毕宇峰
杨峥
YAN Xue-zhen;QU Qiang-qiang;HUANG Li-ping;BI Yu-feng;YANG Zheng(Acupuncture Department,Shaanxi Traditional Chinese Medicine Hospital,Xi'an 710000,China)
出处
《临床医学研究与实践》
2020年第1期129-131,134,共4页
Clinical Research and Practice
基金
陕西省中医药管理局项目(No.15-LC022)
陕西省自然科学基础研究计划项目(No.2017JM8177)
