摘要
[Objectives]To study the culture of callus produced by the leaves of the ethnic medicine‘E Se’,the induction of adventitious buds and the regeneration system of tissue culture seedlings.[Methods]Multi-factor orthogonal experiment was used to design and analyze.[Results]The best medium for callus induction was MS+2.0 mg/L 6-BA+0.1 mg/L 2,4-D.By comparing the effects of cytokinin 6-BA,KT and TDZ on induction of adventitious bud differentiation,it was found that the optimum medium for adventitious bud regeneration was MS+0.5 mg/L 6-BA+0.5 mg/L KT+0.15 mg/L IBA.Finally,adding 0.2 mg/L IBA(0.1 or 0.2 mg/L)to MS medium could effectively make regenerated seedlings produce roots,and the efficiency was more than 70%.[Conclusions]It laid a foundation for the large-scale cultivation of the seedlings of the ethnic medicine‘E Se’and the promotion of their artificial cultivation.
[Objectives] To study the culture of callus produced by the leaves of the ethnic medicine ‘E Se’, the induction of adventitious buds and the regeneration system of tissue culture seedlings. [Methods] Multi-factor orthogonal experiment was used to design and analyze. [Results] The best medium for callus induction was MS+2.0 mg/L 6-BA+0.1 mg/L 2, 4-D. By comparing the effects of cytokinin 6-BA, KT and TDZ on induction of adventitious bud differentiation, it was found that the optimum medium for adventitious bud regeneration was MS+0.5 mg/L 6-BA+0.5 mg/L KT+0.15 mg/L IBA. Finally, adding 0.2 mg/L IBA(0.1 or 0.2 mg/L) to MS medium could effectively make regenerated seedlings produce roots, and the efficiency was more than 70%. [Conclusions]It laid a foundation for the large-scale cultivation of the seedlings of the ethnic medicine ‘E Se’ and the promotion of their artificial cultivation.
基金
Supported by Science and Technology Program of Sichuan Province(2018NZ0091)
Special Project of Sichuan Provincial Public-Interest Scientific Institution Basal Research Fund
Open Fund for Key Laboratory of Coarse Cereal Processing,Ministry of Agriculture and Rural Affairs(2018CC12)
Scientific Research Projects of Sichuan Province Traditional Chinese Medicine Administration(2018KF007)
