摘要
通过吸附和解吸附实验,考察并优化了变叶海棠叶中总黄酮的纯化工艺及工艺参数。通过比较12种不同极性的大孔吸附树脂对变叶海棠叶总黄酮的吸附、解吸附能力,最终选取AB-8树脂进行实验。结果表明,最优静态吸附、解吸附工艺参数为:pH=4下静态吸附3h;体积分数为70%的乙醇(pH=7)静态解吸附3h;最优动态吸附、解吸附工艺参数:以2 BV/h的流速上样9 BV(柱体积)样品溶液,随后在2 BV/h流速下,分别以6 BV水、6 BV体积分数为20%的乙醇、6 BV体积分数为40%的乙醇、6 BV体积分数为60%的乙醇、6 BV体积分数80%的乙醇对上样柱进行洗脱,在此条件下分离纯化,变叶海棠叶总黄酮质量分数由32.79%提高到59.18%。红外光谱显示变叶海棠叶中具备黄酮类物质特征官能团,证明黄酮类物质的存在。对精制后的变叶海棠叶总黄酮进行体外抗炎作用考察,在50~400mg/L质量浓度下,变叶海棠叶总黄酮对脂多糖诱导巨噬细胞炎症反应有明显的抑制作用,可能是通过抑制与炎症相关的基因表达来抑制NO、IL-6、IL-1β、TNF-α的分泌。
The purpose of this research was to study the purification process of flavonoids in Malus toringoides(Rehd.) Hughes leaves(FMTs), adsorption and desorptiontests optimised the purification parameters. AB-8 resin was selected by comparing the adsorption and desorption ability of FMTs on twelve types of resins. The results showed that the optimal purification parameters of static adsorption and desorption were the sample at pH=4 with 3 h of adsorption;the desorption solvent was 70% ethanol(volume fraction, pH=7.0) with 3 h of desorption. The best dynamic parameters were a sample loading amount of 9 bed volumes(BV) at a feeding flow rate of 2 BV/h and washing the column with 6 BV of water, followed by subsequent flushing with 20%(6 BV), 40%(6 BV), 60%(6 BV) and 80%(6 BV) ethanol in water(volume fraction) at an elution flow rate of 2 BV/h. Under these conditions, the mass fraction of FMTs increased from 32.79% to 59.18%. The existence of flavonoids was confirmed by their characteristic functional groups that were identified by infrared spectroscopy. Meanwhile, FMTs in the dosage range of 50 to 400 mg/L showed a significant anti-inflammatory effect on lipopolysaccharide(LPS)-induced RAW 264.7 mouse macrophage cells, which could inhibit the secretion of NO, IL-6, IL-1β and TNF-αthrough the inhibition of inflammatory-related gene expression.
作者
常柄权
李斌
袁瑞瑛
卓玛东智
黄山
CHANG Bing-quan;LI Bin;YUAN Rui-ying;ZHUO MADong-zhi;HUANG shan(Department of Pharmacy,College of Chemical Engineering,Qingdao University of Science and Technology,Qingdao 2660 42,Shandong China;Department of Medicament,College of Medicine,Tibet University,Lhasa 850000,Xizang,China)
出处
《精细化工》
EI
CAS
CSCD
北大核心
2019年第6期1149-1158,共10页
Fine Chemicals
基金
National Natural Science Foundation of China(81760779)
Natural Science Foundation of Xizang(Tibet)Autonomous Region(XZ2017ZRG-07)~~
关键词
变叶海棠叶
黄酮
大孔吸附树脂
抗炎活性
医药与日化原料
Malus toringoides(Rehd.)Hughes
leaves
Flavonoids
Macroporous resins
Anti-inflammation activities
Drug and cosmetic materials
