摘要
目的:利用高效液相色谱技术,建立前胡及其易混用品紫花前胡和硬前胡的特征图谱,结合主成分分析,用于该类药材的鉴别和质量控制。方法:采用Aglient TC C18色谱柱(4.5 mm×250 mm,5μm),以水(A)-甲醇(B)为流动相,梯度洗脱(0~15 min,40%B;15~16 min,40%B→48%B;16~26 min,48%B→75%B;26~55 min,75%B→95%B;55~56 min,95%B→99%B;56~60 min,99%B,流速1.0mL·min^-1,检测波长325 nm,柱温30℃,进样量5μL,分别建立不同产地前胡、紫花前胡、硬前胡的HPLC特征图谱,并通过Chempattern及Ezinfo化学计量学软件对所采集的数据进行主成分分析。结果:主成分分析结果显示前胡及其易混用品分别聚在不同的区域,所含化学成分存在明显差异。结论:本方法稳定性、重现性好,所建立的特征图谱专属性强,可用于前胡及其易混用品的鉴别和质量控制。
Objective:To establish HPLC characteristic fingerprints of Peucedani Radix and its promiscuous herbs(Peucedani Decursivi Radix and Peucedani Subglabri Radix),and to identify and control the quality of the medicinal materials by principle component analysis(PC A).Methods:The HPLC analysis was carried out on an Aglient TC-C18 column(4.6 mm × 250 mm,5 μm)with water(A)-methanol(B)as mobile phase by gradient elution(0-15 min,40%B;15-16 min,40%B→48%B;16-26 min,48%B→75%B;26-55 min,75%B→95%B;55-56 min,95%B→99%B;56-60 min,99%B),at a flow rate of 1.0 mL·min^-1. The column temperature was set at30 ℃,the detection wavelength was set at 325 nm,and the injection volume was 5 μL.HPLC characteristic fingerprints of Peucedani Radix,Peucedani Decursivi Radix and Peucedani Sub glabri Radix from different habitats were established,respectively.The obtained data were analyzed by Chempattern and Ezinfo chemometrics software.Results:PC A were used to analyze the LC-MS data,the results showed that Peucedani Radix,Peucedani Decursivi Radix and Peucedani Subglabri Radix clustered in different zones respectively,and chemical compositions in Peucedani Radix and its promiscuous herbs varied obviously.Conclusion:The proposed method is stable and reproducible,and the established characteristic fingerprint is specific,which can be used for identification and quality control of Peucedani Radix and its promiscuous herbs.
作者
李运
邱国玉
李晓春
程显隆
魏锋
马双成
LI Yun;QIU Guo-yu;LI Xiao-chun;CHENG Xian-long;WEI Feng;MA Shuang-cheng(Lanzhou Institute for Food and Drug Control,Lanzhou 730050,China;National Institutes forFood and Drug Control,Beijing 100050,China)
出处
《药物分析杂志》
CAS
CSCD
北大核心
2019年第7期1323-1329,共7页
Chinese Journal of Pharmaceutical Analysis
基金
甘肃省食品药品监督管理局2018年度食品药品科研项目(2018GSFDA042)
兰州市2019年第一批科技计划项目(2019-1-60)
关键词
白花前胡
紫花前胡
少毛北前胡
香豆素类化合物
紫花前胡苷
白花前胡甲素
白花前胡乙素
白花前胡E素
主成分分析
特征图谱
Peucedanum praeruptorum
Peucedanum decursivum
Peucedanum harry-smithii Fedd.ex Wolff var.subglabrum
coumarins
nodakenin
praeruptorin A
praeruptorin B
praeruptorin E
principle component analysis
characteristic chromatogram
