摘要
目的:通过对人工栽培与野生白及进行的主成分含量测定、特征图谱与PLS-DA相结合的多元统计分析,揭示了两者之间的化学成分存在差异。方法:采用Thermo Hypersil Gold Aq色谱柱(4.6 mm×250mm,5μm),以乙腈为流动相A,0.05%磷酸水溶液为流动相B,梯度洗脱,流速1.0 mL·min-1,柱温30℃,检测波长为280 nm,进样量20μL。对白及的主要成分四裂红门兰素进行含量测定,并建立其特征图谱,通过ChemPattern软件确立共有峰,将共有峰面积信息导入Simca-P软件进行多元统计分析。结果:人工栽培白及的四裂红门兰素含量高于野生,通过特征图谱确定7个共有峰,在PLS-DA得分图第一主成分上两者能够明显区分开。结论:人工栽培与野生白及存在化学成分的差异,并且能通过多元统计分析加以区分。
Objective:To reveal the different chemical components between the cultivated and wild Bletillae Rhizoma,the principal component analysis,characteristic chromatogram and PLS-DA coupled with multivariate statistical analysis were conducted. Methods:Separation was performed on a Thermo Hypersil Gold Aq(4.6 mm×250 mm,5 μm)column with gradient elution of acetonitrile and 0.05% phosphate acid at a flow rate of 1.0 mL·min-1. The column temperature was 30 ℃ and the injection volume was 20 μL. Common peaks were analyzed by ChemPattern software,and the multivariate statistical analysis of common peak area was performed by Simca-P. Results:The results showed that the contents of militarine in cultivated Bletillae Rhizoma were higher than those of the wild group. In the characteristic chromatogram,7 common peaks were confirmed. In the PLSDA score plot,the cultivated Bletillae Rhizoma could be clearly separated from wild group in the PC1 direction. Conclusion:Our findings reveal the obvious differences of chemical components among the cultivated and wild Bletillae Rhizoma,which can be distinguished by multivariate statistical analysis.
作者
朱环
张丽萍
王江聪
谢秉湘
谢循策
张崇生
ZHU Huan;ZHANG Li-ping;WANG Jiang-cong;XIE Bing-xiang;XIE Xun-ce;ZHANG Chong-sheng(Wenzhou Institute for Food and Drug Control,Wenzhou 325000,China)
出处
《药物分析杂志》
CAS
CSCD
北大核心
2020年第6期1045-1049,共5页
Chinese Journal of Pharmaceutical Analysis
