摘要
目的 动态观察壳聚糖管与聚乙醇酸纤维复合移植修复大鼠坐骨神经缺损的过程及结果。 方法 用复合移植物辅加“神经再生素”桥接大鼠坐骨神经 10mm的缺损 ,分别于术后不同时间行足迹实验及再生神经的免疫组化、光电镜观察。 结果 术后 1周移植物表面即可见纤维膜性组织和微血管形成 ,第 4周再生神经到达缺损的远段 ,第 8周部分神经再生通过缺损 ,随时间延长 ,有髓神经纤维比例增多 ,髓鞘增厚。第 16周 ,移植物基本降解 ,被再生神经组织替代。足迹试验显示术后第 8周坐骨神经功能开始恢复 ,第 16周达正常的 60 %左右。 结论 复合移植物可诱导许旺细胞的迁移和再生轴索的生长 ,有利于神经再生时各种膜性结构及微血管形成 ,并在发挥“桥梁”作用后逐渐降解。
Objectives To investigate the process and the result of the repair of the sciatic nerve in rat by chitosan tube and polyglycolic fiber Methods The left sciatic nerves of rats were transected with 10 mm gap, then repaired with the nerve grafts in combination of NRF Walking track analysis, immunohistochemical and transmission electron microscopic examinations were carried out at different time after the operation Results Fibrous membrane and capillary could be observed on the surface of the grafts at the first week after the operation; at the 4th week, the regenerated nerve fibers grew more than half of the distances of the gap; at the 8th week, regenerated nerve fibers overcame the gap, many myelinated nerve fibers and thick myelin sheathes could be observed; at the 16th week, the grafts almost degraded and were substituted by the regenerated nerve Walking track analysis showed that sciatic nerve function recovery appeared at the 8th week, and that at the 16th week, approximately 60% of the sciatic nerve function was recovered Conclusion The nerve graft with NRF can stimulate the growth of vessels and the formation of the fibrous membrane, and is useful to the migration of Schwann cells and regeneration of axons, and degrade after the nerve regeneration
出处
《中华显微外科杂志》
CSCD
北大核心
2002年第4期274-277,共4页
Chinese Journal of Microsurgery
基金
国家自然科学基金项目 (30 0 70 2 55)
