摘要
用不同剂量的几丁糖溶液分别加入人成纤维细胞和表皮角化细胞于细胞培养液中,培养72小时,测定细胞数。结果:人成纤维细胞数随着几丁糖溶液剂量的增加而减少;人表皮角化细胞数随着几丁糖溶液剂量的增加,在一定范置内相应增加。从而肯定丁几丁糖溶液能抑制人成纤维细胞生长,促进人表皮角化细胞生长。
Human fibroblasts and human epidermal keratinocytes were used for culture. Chitosan solution were added in the culture solution(DMEM). After 72 hours, the fibroblasts showed rapid growth in the control culture without Chitosan, But the numbers of human fibroblasts from growth was decreased as the concentration of Chitosan was increasing. On the contrary the human epidermal keratinocytes growed more rapidly in the culture with Chitosan than in the culture without Chitosan. The results showed that Chitosan inhibited the growth of human fibroblast and stimulated the growth of human epidermal keratinocyte.
出处
《中国修复重建外科杂志》
CAS
CSCD
1993年第4期244-245,共2页
Chinese Journal of Reparative and Reconstructive Surgery
关键词
几丁糖
细胞培养
细胞
生物材料
Human epidermal keratinocyte Human fibroblast Chitosan Culture
