摘要
目的:从新生大鼠海马分离培养并鉴定神经干细胞。方法:应用含有碱性成纤维细胞生长因子(bFGF)和表皮生长因子(EGF)的无血清条件培养基,采用无底物的悬浮培养法培养神经干细胞,并用免疫细胞化学技术鉴定传代后形成的细胞团。结果:EGF+bFGF可以明显地促进神经干细胞分裂增殖,免疫细胞化学检测发现传代后形成的细胞团中的细胞均表达神经上皮干细胞蛋白(Nestin)。结论:我们成功地建立了新生大鼠海马神经干细胞培养模型。
Objective: To isolation ,culture and identify the neural stem cells from the hippocampus of the newborn rats. Methods: The neural stem cells were isolated from the hippocampus of the newborn rats and cultured by using serum-free medium containing bFGF and EGF without the substrate. After the neural stem cells gave rise to neuropheres,they were mechanically dissociated to single cells,and after the single cells gave rise to neuropheres again which were identified by immunocytochemistry. Result: Neural stem cells isolated from the hippocampus of the newborn rats could be maintained and propagated in the present of EGF and bFGF .The cells in the neuropheres were identified as neural stem cells by immunocytochemistry,because they expressed nestin which is a specific marker of neural stem cells. Conclusion: The culture method of neural stem cells from the hippocampus of the newborn rats was successfully founded.
出处
《黑龙江医药科学》
2002年第5期2-4,共3页
Heilongjiang Medicine and Pharmacy
基金
国家自然科学基金资助项目(批准号30170963)
