摘要
利用蚕蛹多肽为原料制备多肽螯合钙,以可溶性钙含量为主要指标,综合考察螯合率和得率,研究了螯合pH、温度、时间以及肽钙比等单因素的影响,并进一步通过响应面优化法对制备工艺进行优化,确定最优的反应条件为反应时间80min、反应温度73℃、pH 10、肽钙质量比3.4:1.0,该条件下蚕蛹多肽螯合钙的含钙量为14.51%。傅利叶红外光谱分析表明蚕蛹多肽主要通过羧基、胺基和磷酸基团与钙离子结合。
Preparation of peptides chelated calcium from silkworm pupapeptides,taking the soluble calcium content as the main index,and considering the chelation and yield rate,the effects of reaction pH,temperature,time,and peptide-calcium ratio were examined.On the basis of single factor experiment,Box-Behnken central composite design was utilized,and the optimal conditions were as followed:reaction time 80 min,reaction temperature73℃,pH 10,peptide calcium ratio 3.4:1.0.Under this condition,the soluble calcium content of the silkworm pupapeptide chelated calcium was 14.51%.Through the UV absorption andinfrared spectroscopy and SEM analysis,it showed that the silkworm pupapeptides and calcium ions chelation occurred,and the phosphate group,carboxyl group and amino group participated in the reaction.
作者
赵梓月
王思远
廖森泰
穆利霞
邹宇晓
ZHAO Zi-yue;WANG Si-yuan;LIAO Sen-tai;MU Li-xia;ZOU Yu-xiao(Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences, Guangzhou,Guangdong 510610, China;Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou,Guangdong 510610, China;Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs,Guangzhou, Guangdong 510610, China)
出处
《食品与机械》
北大核心
2019年第8期20-26,共7页
Food and Machinery
基金
现代农业产业技术体系建设专项(编号:CARS-18)
广东省现代农业产业技术体系创新团队项目(编号:2016LM1087)
广州市科技计划项目(编号:201804010095)
关键词
蚕蛹多肽
螯合反应
补钙制剂
结构表征
silkworm pupapeptides
chelation reaction
calcium supplement
structure characterization
