摘要
目的分析云南省新发现的1个HIV-1独特型重组毒株的基因结构和重组特点。方法在云南省2016年HIV基因型耐药检测样本中,发现1例患者体内HIV-1毒株pol区存在重组片段,运用RT-PCR的方法分两段扩增病毒近全长基因组并测定序列。使用RIP、jpHMM和SimPlot3.5软件进行重组分析,同时采用MEGA6.06软件共同构建Neighbor-joining系统进化树对该毒株的同源关系进行分析。结果共获得长度为8 590 bp的HIV-1近全长基因序列,断点分析表明该序列由CRF01_AE、B和C亚型片段组成,其中CRF01_AE作为骨架,插入B和C亚型片段,对应HIV-1 HXB2的位置分别是791~1 171为CRF01_AE,1 172~2 652为C,2 653~2 977为B,2 978~9 380为CRF01_AE。结论云南省近年来发现了部分由CRF01_AE、B和C亚型交叉重组形成的新型毒株,而本研究发现的这个毒株重组模式复杂,提示我们应密切监测流行趋势变化,这对于了解当前HIV-1流行情况和变化趋势具有重要意义。
Objective To analyze the genetic structure and recombination characteristics of a newly discovered HIV-1 unique recombinant strain in Yunnan Province. Methods During a test for drug-resistant HIV genotypes in Yunnan Province in 2016, a recombinant fragment was found in the pol region of a HIV-1 strain isolated from a patient. Two overlapping segments of the HIV-1 genome were amplified by RT-PCR, and then the products were sequenced. Recombination analysis was performed using RIP, jpHMM and SimPlot3.5 software. A phylogenetic tree was constructed for homology analysis by Neighbor-joining method using MEGA6.06 software. Results A nearly full-length HIV-1 gene sequence with 8 590 bp in length was obtained. Breakpoint analysis indicated that the sequence consisted of CRF01_AE and fragments of B and C subtypes. CRF01_AE was used as the backbone with B and C subtype fragments inserted. The positions were 791 to 1 171 for CRF01_AE, 1 172 to 2 652 for C subtype fragment, 2 653 to 2 977 for B subtype fragment, and 2 978 to 9 380 for CRF01_AE using HIV-1 HXB2 as the reference strain. Conclusions Some new strains formed by cross-recombination of CRF01_AE and B and C subtypes were discovered in Yunnan Province in recent years. It was found that the recombination pattern of the newly discovered strain was complex, suggesting that close attention should be paid to the changes in epidemic trends, which was of great importance to understand the current prevalence and epidemic trends of HIV-1.
作者
刘家法
张米
杨壁珲
邓雪媚
孙艾丝
雷素云
李健健
Liu Jiafa;Zhang Mi;Yang Bihui;Deng Xuemei;Sun Aisi;Lei Suyun;Li Jianjian(Department of Laboratory, Yunnan Provincial Infectious Diseases Hospital/AIDS Care Center, Kunming 650301, China)
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2019年第8期601-607,共7页
Chinese Journal of Microbiology and Immunology
基金
十三五国家科技重大专项(2018ZX10721102-002)
云南省医疗卫生单位内设研究机构项目(2017NS124)
云南省教育厅科学研究基金项目(2018JS252).
关键词
人类免疫缺陷病毒1型
独特型重组
近全长基因序列分析
Human immunodeficiency virus type 1
Unique recombinant form
Near-full-length gene sequence analysis
