摘要
实验设计了6个不同维生素D3水平(0,1000IU·kg^-1,2000IU·kg^-1,4000IU·kg^-1,8000IU·kg^-1,100000IU·kg^-1)的等氮等能饲料(组1、组2、组3、组4、组5和组6)来研究斜带石斑鱼对维生素D3的需求量。结果显示,摄食组3饲料的斜带石斑鱼增重率、特定生长率以及饲料效率显著高于其他组(P<0.05)。不同水平的维生素D3不影响石斑鱼的肥满度和肝体比,添加量大于2000IU·kg^-1时,石斑鱼肠系膜脂肪和脏体比随着维生素D3水平的升高而降低(P<0.05)。鱼体肌肉和全鱼中蛋白含量随着维生素D3水平的升高而降低,粗脂肪含量各组之间没有显著性差异。维生素D3添加量低于2000IU·kg^-1时,石斑鱼骨骼中钙(Ca)、磷(P)含量与维生素D3的添加量呈正相关。以增重率以及骨骼Ca、P含量为指标,斜带石斑鱼幼鱼实用饲料中维生素D3的适宜添加量为2000IU·kg.1。
Six isonitrogenous and isoenergetic diets supplemented with different levels of vitamin D3 (0, 1 000 IU·kg^-1, 2 000 IU·kg^-1, 4 000 IU·kg^-1, 8 000 IU·kg^-1, 100 000 IU·kg^-1) were formulated to evaluate the requirement of vitamin D3 to Epinephelus coioides, namely Diet 1, Diet 2, Diet 3, Diet 4, Diet 5 and Diet 6. Diet 3 obtained higher weight gain rate, specific growth rate and feed efficiency than the other groups (P<0.05). With dietary supplemental vitamin D3 higher than 2 000 IU·kg.1, the intraperitoneal fat ratio (IPF) and viscerosomatic index (VSI) decreased significantly with increasing dietary vitamin D3 levels (P<0.05). The protein contents in whole body and muscle decreased with increasing dietary vitamin D3 levels (P<0.05), but the lipid and ash contents showed no difference in whole body and muscle among all the groups. When the dietary supplemental vitamin D3 was lower than 2 000 IU·kg^-1, the calcium (Ca) and phosphorus (P) contents in fish bone were positively correlated with the vitamin D3 levels. Based on Ca and P contents in bones, the optimal supplementation level of vitamin D3 in practical diet should be 2 000 IU·kg^-1.
作者
谢诗玮
田丽霞
刘永坚
张海涛
牛津
梁键钧
方蔚平
苏积财
XIE Shiwei;TIAN Lixia;LIU Yongjian;ZHANG Haitao;NIU Jin;LIANG Jianjun;FANG Weiping;SU Jicai(Fish Nutrition Laboratory,College of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China;Guangdong Hengxing Feed Industry Co.,Ltd.,Zhanjiang 524000,China;Zhuahi Dehai Biotechnology Co.,Ltd.,Zhuhai 519100,China)
出处
《南方水产科学》
CAS
CSCD
北大核心
2019年第4期61-67,共7页
South China Fisheries Science
基金
湛江市科技计划项目(2016A02004)
国家自然科学基金面上项目(31672665)
关键词
斜带石斑鱼
维生素D3
营养需要
体成分
Epinephelus coioides
vitamin D3
nutrition requirement
body composition
