摘要
研究饲料中添加维生素D3对黄颡鱼（Pelteobagrus fulvidraco）幼鱼血清和肝脏抗氧化功能以及血清补体C3、C4含量的影响。试验选取黄颡鱼（2.0±0.0）g 900尾,随机分为6组,每组设3缸重复,每缸50尾鱼。基础饲料以酪蛋白和明胶为蛋白源,糊精为糖源,豆油为脂肪源。在基础饲料中添加包膜饲料级维生素D3配制成维生素D3含量分别为0,324,1 243,3 621,8 040和22 700 IU/kg的6组饲料。试验持续12周。结果显示,与对照组相比,维生素D3添加组血清和肝脏超氧化物歧化酶（SOD）活性显著提高（P〈0.05）,而肝脏脂质过氧化物（LPO）含量显著降低（P〈0.05）,但维生素D3最高剂量添加组LPO含量升高。各维生素D3添加组肝脏过氧化氢酶（CAT）活性呈先升高后降低趋势（P〈0.05）,其中维生素D3含量为3 621 IU/kg组活性最高。血清CAT活性、C3含量、C4含量,血清和肝脏丙二醛（MDA）含量不受饲料中维生素D3含量影响。结果表明,维生素D3含量为324-3 621 IU/kg的饲料可提高黄颡鱼幼鱼抗氧化能力。
This experiment was conducted to investigate the effects of dietary vitamin D3 supplementation on the serum and liver antioxidant capacities and serum complement C3 and complement C4 contents in juvenile Pelteobagrus fulvidraco. For the basal diet, casein and gelatin were used as protein sources, dextrin as the carbohydrate source, and soybean oil as the lipid source. Six experimental diets were formulated to contain graded levels of vitamin D3 （0, 324, 124 3, 362 1, 804 0 and 227 00 IU/kg, respectively）. Each diet was assigned to triplicate tanks of 50 juvenile P.fulvidraco （2. 0 ±0. 0 g） for 12 weeks. The results were as follows： vitamin D3 supplementation significantly improved activities of superoxide dismutase （SOD） in serum and liver （ P 〈 0.05 ）, accompanying with decrease of liver lipid hydroperoxide （LPO） level （ P 〈0. 05） , however, fish fed the highest amount of vitamin D3 tended to have higher liver LPO level. Among the vitamin D3 addition groups, liver catalase （CAT） activity increased from 324 IU/kg group to 362 1 IU/kg group and then decreased （P 〈 0.05）. Dietary vitamin D3 had no effect on serum CAT activities and serum complement C3, serum complement C4, serum MDA and liver MDA contents. In conclusion, vitamin D3 addition improved antioxidant eapacity and health condition of juvenile P.fulvidraco, and the recommended vitamin D3 level in the diet was 324 -362 1 IU/kg.
出处
《淡水渔业》
CSCD
北大核心
2014年第3期80-84,共5页
Freshwater Fisheries
基金
国家自然科学基金(30901105和31172421)
现代农业产业技术体系建设专项资金(CARS-46)
中央高校基本科研业务费专项资金(2013PY076)
