摘要
目的为满足颌面部骨缺损修复的需要,文中旨在构建氧化石墨烯(GO)修饰的三维联通纳米氧化锆(ZrO2)骨组织工程支架,并评价GO修饰后复合支架的表面形貌、机械强度以及细胞相容性。方法采用改良Hummers法制备GO,扫描电镜、透射电镜及傅里叶红外光谱进行表征。硅烷介导下,将ZrO2支架与不同浓度(0.5、1、1.5mg/mL)GO分散液结合,选择表面均匀结合GO的复合支架进行压缩强度测试,并与人牙髓干细胞(hDPSCs)共培养,肌动蛋白染色观察细胞在支架上生长情况,MTS法检测细胞活力。结果表征结果显示:扫描电镜下观察,GO呈片状,可见褶皱的表面形貌,部分GO片层在边缘折叠翘起;透射电镜下可清晰地观察到GO呈轻纱状半透明微皱的片状结构,高分辨过滤像中可见该区域的晶体结构显示出与石墨一样的六元环结构。高倍电镜下1.0mg/mLGO-ZrO2支架可见到薄层的GO沉积在支架骨架的裂纹处,连接裂纹两端,并且可观察到陶瓷颗粒表面沉积带有褶皱的层状GO。机械性能对比结果显示,GO-ZrO2支架的压缩强度为([1.292±0.087)Mpa]较ZrO2支架([1.031±0.076)Mpa]显著增加(P<0.05)。与单纯ZrO2支架相比,在GO-ZrO2支架上均可见细胞更为密集的伸展黏附于支架表面,表现出更为活跃的细胞增殖状态。细胞活力检测结果显示,hDPSCs在GO-ZrO2支架上培养1、3、5d时,细胞活力均显著高于单纯ZrO2支架(P<0.05)。结论GO修饰ZrO2支架可改善机械性能,促进hDPSCs的早期增殖,呈现出良好的细胞相容性。
Objective In order to meet the needs of maxillofacical bone defect repair,the aim of this study was to synthesize graphene oxide(GO)modified three-dimensional conneted nano- zirconia(ZrO2)bone tissue engineering scaffold and evaluate its surface morphology,compressive strength and cytocompatibility. Methods GO was synthesized by a modified Hummers method and then was testified by scanning electron microscope,transmission electron microscopy and fourier transform infrared spectroscopy. ZrO2 scaffold was modified by different concentrations(0.5,1.0,1.5mg/ mL)of GO dispersion via a silane-mediated method. The composite scaffold with uniform GO coating was chosen for compressive strength test and co-cultured with human dental pulp stem cells(hDPSCs). Actin staining was used to observe the growth of the cells on the scaffold,and MTS was used to detect the cell activity. Results The characterization results showed that,under scanning electron micro. scope,the GO was flaky and the surface morphology of folds could be seen. Part of the GO layer folds up at the edge. Under transmission electron microscopy,the GO was clearly observed to have a gossylike,translucent and slightly wrinkled lamellar structure. The crystal structure in this area in the high-resolution filter image showed a six-member ring structure like graphite. Under high power electron microscope,the 1.0mg/ml GO-ZrO2 scaffold could be seen to deposit a thin layer of GO at the crack of the scaffold skeleton,connecting the two ends of the crack,and lamellar GO with folds could be observed on the surface of ceramic particles. The comparison of mechanical properties showed that the compression strength of GO-ZrO2 scaffold was sgnificantly increased compared with that of ZrO2 scaffold[(1.292±0.087)vs(1.031±0.076),P<0.05]. Compared with the simple ZrO2 scaffold,the GO-ZrO2 scaffold showed more dense extension and adhesion to the surface of scaffolds,showing more active cell proliferation. The cell viability test showed that the viability of hDPSCs was significantly improved on GO-ZrO2 scaffold after 1,3 and 5 days of proliferation compared with the simple ZrO2 scaffold(P<0.05). Conclusion The ZrO2 scaffold modified by GO improved compressive strength,promoted the early proliferation of hDPSCs with good cytocompatibility.
作者
魏小翠
穆睿
毕博
韩璧瑶
于泓川
陈博
王玥
史小蕾
臧圣奇
金磊
WEI Xiao-cui;MU Rui;BI Bo;HAN Bi-yao;YU Hong-chuan;CHEN Bo;WANG Yue;SHI Xiao-lei;ZANGSheng-qi;JIN Lei(Department of Stomatology,Jinling Hospital,Nanjing School Clinical Medicine,Southern Medical University/General Hospital of Eastern Theater Command,PLA,Nanjing 210002,Jiangsu,China)
出处
《医学研究生学报》
CAS
北大核心
2019年第8期797-802,共6页
Journal of Medical Postgraduates
基金
国家自然科学基金(81771992)
关键词
氧化石墨烯
氧化锆支架
牙髓干细胞
骨组织工程
graphene oxide
Zirconia scaffold
dental pulp stem cells
bone tissue engineering
