摘要
目的:研究人类白细胞抗原E(HLA-E)是否参与孕激素对JEG-3细胞VEGF基因表达的调节作用。方法:JEG-3细胞分为5组,接受不同处理:空白对照(组1)、孕激素处理(组2)、转染HLA-EsiRNA慢病毒后孕激素处理(组3),HLA-EsiRNA慢病毒转染(组4)以及慢病毒阴性对照转染(组5)。48小时后收集细胞,分别采用Real-timePCR和Westernblot方法检测JEG-3细胞血管内皮生长因子(VEGF)mRNA及蛋白表达水平。结果:组2JEG-3细胞的VEGFmRNA和蛋白表达较组1增高,差异有统计学意义(P<0.05);沉默HLA-E基因(组4)导致VEGFmRNA和蛋白表达降低,与组1比较,差异有统计学意义(P<0.05)。组3JEG-3细胞经HLA-EsiRNA慢病毒转染、沉默HLA-E表达后再接受孕激素处理,VEGFmRNA及蛋白表达水平与组1比较差异无统计学意义(P>0.05)。组5与组1比较,VEGFmRNA及蛋白水平比较,差异无统计学意义(P>0.05)。结论:HLA-E介导孕激素对JEG-3滋养细胞VEGF表达的上调,孕激素对JEG-3细胞VEGF的上调作用被HLA-E基因的沉默消除。
Objective: To determine if human leukocyte antigen( HLA)-E played a role in the effect of progesterone on the Vascular endothelial growth factor( VEGF) expression in chorion carcinoma JEG-3 cells. Methods: JEG-3 cells were allocated into five groups including group 1 ( the blank control),group 2( progesterone treatment),group 3( transfection of lentivirus carrying siRNA targeting HLA-E before progesterone treatment),group 4 ( transfection of lentivirus carrying siRNA targeting HLA-E),and group 5( transfection of the negative control siR- NA).The mRNA and protein levels of VEGF in JEG-3 cells were detected by real-time quantitative PCR and Western blot assay,respectively 48 h later.Results: The expression level of VEGF mRNA and protein in JEG-3 was up-regulated after treatment of progesterone( group 2) while down-regulated after transfected with lentivirus carrying siRNA targeting HLA-E( group 4),both with significant differences compared to the control group( P< 0. 05).The HLA-E in JEG-3 was silenced by transfection of siRNA targeting HLA-E before progesterone treatment,with no difference in VEGF mRNA and protein expression level compared to the control group.Conclusions: HLA-E is involved in the expression level of VEGF and diminishes the up-regulatory effect of progesterone on VEGF in JEG-3.
作者
陈悦悦
黄仲英
樊伟
马黔红
CHEN Yueyue;HUANG Zhongying;FAN wei(Department of Obstetrics and Gynecology,West China Second Hospital,Sichuan University,Key Laboratory of Birth Defects and Related Diseases of Women and Children,Ministry of Education,Chengdu Sichuan 610041,China)
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2019年第7期514-517,共4页
Journal of Practical Obstetrics and Gynecology
基金
国家自然科学基金(编号:81200453)
关键词
人类白细胞抗原E
孕激素
滋养细胞
血管内皮生长因子
Human leukocyte antigen E
Progesterone
Trophoblast cell
Vascular endothelial growth factor
