摘要
目的:研究人类白细胞抗原E(HLA-E)在肝癌细胞系中的表达情况。方法:通过real-time PCR和Western blotting技术研究5种肝癌细胞系和胎肝细胞系中HLA-E mRNA和蛋白的表达情况,进行相对定量分析。结果:Real-time PCR检测显示,HepG2细胞、Bel7402细胞、PLC细胞、MHCC97细胞和Hep3B2.1-7细胞这5种肝癌细胞系与L02胎肝细胞系的HLA-E mRNA水平比较,除Hep3B2.1-7细胞表达几乎接近缺失(P<0.01)外,其余无显著差异(P>0.05)。HepG2细胞、Bel7402细胞、PLC细胞、MHCC97细胞和Hep3B2.1-7细胞与L02细胞的HLA-E蛋白水平比较,差异显著(P<0.01),其中Hep3B2.1-7细胞表达缺失。结论:肝癌细胞系的HLA-E mRNA和蛋白表达不同步,可能存在转录后调控。
AIM： To investigate the human leukcyte antigen E （HLA- E） expression in hepatocellular carci- noma cell lines. METHODS： The techniques of real - time PCR and Western blotting were used to study the HLA - E ex- pression in the 5 cell lines of hepatocellular carcinoma and a fetal liver cell line at mRNA and protein levels. RESULTS ： The results of real - time PCR showed that no statistical difference of HLA - E mRNA level between fetal liver cell L02 and other 4 cell lines of hepatocellular carcinoma （ HepG2, Be17402, PLC and MHCC97 ） was observed, and almost absence of HLA- E mRNA expression in Hep3B2.1 -7 cells was detected. However, the results of Western blotting showed that there was a significant statistical difference of HLA - E protein levels between L02 cells and the 5 cell lines of hepatocellular carcinoma （HepG2, Be17402, PLC, MHCC97 and Hep3B2.1 -7）, and no HLA - E protein in Hep3B2.1 -7 cells was detectable. CONCLUSION： Asynchronization of HLA - E expression between mRNA and protein levels was found in hepatocellular carcinoma cell lines.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2012年第10期1884-1886,共3页
Chinese Journal of Pathophysiology
基金
国家重点基础研究发展计划(973分课题)(No.2009CB522404)
国家自然科学基金面上项目(No.30571769
No.30972914)
十一五科技攻关(No.2008ZX10002-025)
广东省科技计划(No.2009A030200006)
教育部新教师基金资助项目(No.20100171120087
No.20100171120084)
广东省自然科学基金博士启动项目(No.10451130001004472)
关键词
人类白细胞抗原E
肝细胞癌
胎肝细胞
基因表达
Human leukocyte antigen E
Hepatocellular carcinoma
Fetal liver cells
Gene expression
