摘要
目的探讨不同分期结肠癌患者血清及肿瘤组织中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和白细胞介素-6(interleukin-6,IL-6)的表达水平及其在结肠癌发病中的意义。方法 30例无肠道疾病患者为对照组,60例结肠癌患者为结肠癌组,其中Dukes分期A/B为早期,C/D为晚期。2组采用ELISA法检测血清TNF-α和IL-6水平,采用免疫组织化学法检测肿瘤患者癌旁组织、肿瘤组织中TNF-α和IL-6表达水平。结果结肠癌晚期患者血清TNF-α[(120.78±80.36)ng/L]、IL-6[(12.78±8.36)ng/L]高于结肠癌早期患者[(70.31±30.03)ng/L、(9.04±5.03)ng/L]和对照组[(10.92±10.01)ng/L、(2.94±1.39)ng/L](P<0.01),结肠癌早期患者高于对照组(P<0.01);早期和晚期结肠癌肿瘤组织TNF-α阳性表达率(43.3%、66.7%)、IL-6阳性表达率(76.7%、80.0%)均明显高于癌旁组织(TNF-α为3.3%、16.7%,IL-6为6.7%、10.0%)(P<0.05),晚期结肠癌肿瘤组织TNF-α、IL-6阳性表达率均高于早期(P<0.05);晚期结肠癌患者血清TNF-α和IL-6表达水平与肿瘤组织中表达水平呈正相关(r=0.65,P=0.00;r=0.52,P=0.00)。结论 TNF-α和IL-6在结肠癌发生、发展中起免疫调节作用,其血清水平检测有一定临床应用价值。
Objective To explore the expressions and significances of tumor necrosis factor-α（TNF α） and interleukin-6 （IL-6） in serum and tumor tissue in different stages of colon cancer. Methods Thirty patients without intestinal diseases were as controls （control group） and 60 patients with colon cancer were as cancer group including patients in early stage （Dukes staging A/B） and advanced stage （Dukes staging C/D）. ELISA technique was used to detect the serum levels of TNF-α and IL-6 in two groups. Immunohistochemistry was used to detect the expressions of TNF-α and IL-6 in cancer tissues and their normal adjacent tissues. Results The serum levels of TNF-α and IL-6 were the highest in advanced-stage patients （（120.78±80. 36）, （12. 78±8. 36） ng/L））, followed by those in early-stage patients （（70. 31±30. 03）, （9.04±5.03） ng/L） and control group （（10.92±10.01）, （2.94±1.39） ng/L） in turns （P〈0.01）. The positive rates of TNF-α （43. 3%, 66. 7%） and IL-6 （76. 7%, 80. 0%） in early- and advanced-stage patients in cancer tissues were significantly higher than those in normal adjacent tissues （TNF-α： 3.3%, 16.7%） ; （IL-6：6.7%, 10.0%）（P〈0.05）, and were significantly higher in advanced-stage patients than those in early-stage patients （P〈0.05）. The expressions of TNF-α and IL-6 in serum were positively correlated with the expressions in cancer tissues in advanced-stage patients （r= 0.65, P=0.00; r=0.52, P=0.00）. Conclusion TNF-αand IL 6 may play immune regulation role in the development and progression of colon cancer and the detection of serum levels has an important value.
出处
《中华实用诊断与治疗杂志》
2015年第10期978-980,共3页
Journal of Chinese Practical Diagnosis and Therapy
基金
国家自然科学基金青年科学基金(81402025)
