摘要
目的探讨青黄散联合健脾补肾方提高骨髓增生异常综合征(MDS)患者外周血红蛋白机制。方法选择2016年2月-2018年2月在中国中医科学院西苑医院及首都医科大学附属北京中医医院血液科就诊的30例MDS患者,采用青黄散联合健脾补肾方治疗6个月。采用血细胞分析仪检测患者治疗前后血常规,亚硝酸氢盐测序法(BSP)检测治疗前后骨髓HDAC1基因甲基化变化,Real-time PCR检测治疗前后HDAC1 mRNA及缺氧诱导因子-1α(HIF-1α)mRNA变化,采用自身前后对照的方法比较治疗前后上述指标的差异。结果治疗前MDS骨髓标本中,HDAC1基因启动子区CpG1、CpG5、CpG12、CpG23位点呈高甲基化,分别为58.67%、85.67%、63.89%、71.63%,治疗后,上述HDAC1基因启动子区4个甲基化位点呈低甲基化状态,分别为0.67%、0、1.33%、1.33%;治疗后HDAC1 mRNA相对表达量较治疗前增加了4.9倍(P<0.01),HIF-1αmRNA相对表达量较治疗前增加了7.5倍(P<0.05)。结论青黄散联合健脾补肾方可通过降低HDAC1基因高甲基化增加下游HIF-1αmRNA表达,发挥提高外周血红蛋白作用。
Objective To investigate the mechanism of Qinghuang Powder combined with Jianpi Bushen Prescription in improving peripheral hemoglobin of myelodysplastic syndromes(MDS) patients. Methods Totally 30 MDS patients were recruited in the hematology department of Xiyuan Hospital of China Academy of Chinese Medical Sciences and Beijing Chinese Medicine Hospital affiliated to Capital Medical University from February 2016 to February 2018. The patients were treated by Qinghuang Powder combined with Jianpi Bushen Prescription for 6 months.Blood cell analyzer was used to determine the blood index before and after treatment, the methylation status of HDAC1 gene and the level of HDAC1 mRNA, HIF-1α mRNA expression in bone marrow were detected by BSP and RT-PCR before and after treatment. Results In 30 cases of MDS bone marrow specimens before treatment, the HDAC1 gene promoter CpG1, CpG5, CpG12 and CpG23 showed high methylation status, which were 58.67%, 85.67%, 63.89%, 71.63% respectively. After treatment, 4 promoter of the HDAC1 gene mentioned above showed low methylation status, they were 0.67%, 0, 1.33%, 1.33% respectively. After treatment, the relative expression of HDAC1 mRNA increased by 4.9 times compared with that before treatment(P<0.01);the relative expression of HIF-1α mRNA increased by 7.5 times compared with that before treatment(P<0.05). Conclusion The mechanism of Qinghuang Powder combined with Jianpi Bushen Prescription for increasing hemoglobin levels is to make HDAC1 gene demethylation which can increase HIF-1α mRNA content.
作者
高飞
许勇钢
王洪志
王德秀
朱千赜
麻柔
GAO Fei;XU Yong-gang;WANG Hong-zhi;WANG De-xiu;ZHU Qian-ze;MA Rou(Oncology Department ,Beijing Chinese Medicine Hospital Affiliated to Capital Medical University ,Beijing 100010;Hematology Department ,Xiyuan Hospital of China Academy of Chinese Medical Sciences ,Beijing 100091)
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2019年第7期810-815,共6页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金青年项目(No.81503516)
北京市自然科学基金面上项目(No.7172100)
