摘要
目的观察青黄散联合健脾补肾中药对骨髓增生异常综合征(MDS)患者骨髓单个核细胞内缺氧诱导因子-1α(hypoxia-inducible factor-1α,HIF-1α)的影响。方法应用流式细胞仪检测25例MDS患者使用青黄散合健脾补肾中药治疗前后骨髓单个核细胞内HIF-1α的变化情况,同时以13名健康人为对照组,检测骨髓各系列细胞HIF-1α变化水平。结果 (1)治疗组25例患者中,有效患者19例,无效患者6例,有效率为76%;(2)与治疗前比较,治疗组治疗后外周血WBC、Hb、PLT、ANC均有明显升高,差异有统计学意义(P<0.05,P<0.01)。(3)与治疗前比较,治疗组患者治疗后骨髓内淋巴细胞、单核细胞、粒细胞、有核红细胞的HIF-1α平均荧光强度增强(P<0.05,P<0.01)。与健康对照组比较,治疗组治疗前骨髓内淋巴细胞、单核细胞、有核红细胞HIF-1α平均荧光强度减弱,粒细胞HIF-1α平均荧光强度明显增强(P<0.01);治疗后HIF-1α平均荧光强度除粒细胞较健康成人组尚有增高外(P<0.01),其余各系差异无统计学意义(P>0.05)。结论青黄散合健脾补肾中药可提高MDS患者骨髓内淋巴细胞、单核细胞、粒细胞、有核红细胞的HIF-1α水平,从而提高MDS患者的Hb水平,改善患者贫血及相关症状。
Objective To study the effect of Qinghuang Powder （QHP） combined Chinese herbs for Pi-strengthening and Shen-reinforcing （CHPSSR） on hypoxia-inducible factor -1α （HIF-1α） in bone marrow mononuclear cells of myelodysplastic syndrome （MDS） patients. Methods Changes of HIF-lcx in bone marrow mononuclear cells of MDS patients were detected in 25 MDS patients treated by QHP combined CHPSSR using flow cytometry. Meanwhile, 13 healthy subjects were recruited as the control group. Changes HIF-1α levels in various serial bone marrow mononuclear cells were detected. Results （1） Among the 25 patients in the treatment group, there were 19 patients effective and 6 patients ineffec- tive, with the total effective rate being 76%. （2） Compared with before treatment, levels of peripheral blood WBC, Hb, PLT, and ANC significantly increased in the treatment group after treatment, showing statistical difference （P 〈0.05, P 〈0.01 ）. （3） Compared with before treatment, the HIF-1α mean fluores- cence intensity was enhanced in bone marrow lymphocytes, monocytes, granulocytes, and nucleated red blood cells of the treatment group after treatment （P 〈0.05, P 〈0.01 ）. Compared with the control group, the HIF-1α mean fluorescence intensity was weakened in bone marrow lymphocytes, monocytes, and nucleated red blood cells of the treatment group before treatment ; while it was obviously enhanced in granulocytes （P 〈0.01 ）. But after treatment the HIF-1α mean fluorescence intensity increased more in the granulocytes of the treatment group than in those of the control group （P 〈0.01 ）, but there was nostatistical difference in bone marrow lymphocytes, monocytes, or nucleated red blood cells. Conclusion QHP combined CHPSSR could improve HIF-1α levels in bone marrow lymphocytes, monocytes, granulo- cytes, and nucleated red blood cells of MDS patients, thus improving Hb levels of MDS patients, and im- proving their anemia and correlated symptoms.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2014年第2期174-178,共5页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.81273931)
关键词
青黄散
健脾补肾中药
骨髓增生异常综合征
缺氧诱导因子-1Α
Qinghuang Powder
Chinese herbs for Pi-strengthening and Shen-reinforcing
myelo-dysplastic syndrome
hypoxia-inducible factor -1α
