摘要
采用酶联免疫吸附法(ELISA)及高效液相色谱—质谱(HPLC-mass spectrometry,HPLC-MS)检测纳豆芽孢杆菌Bacillus natto 16-1各细胞成分对小麦粉中呕吐毒素(deoxynivalenol,DON)的清除效果及降解产物,探讨纳豆芽孢杆菌清除呕吐毒素的效果及降解机制。结果显示,培养5h后不同浓度的菌悬液、发酵上清液、全菌裂解液、粗提酶液以及细胞壁悬浮液,对DON清除率均在53%以上,其中不同浓度的菌悬液处理间存在显著差异,高浓度细胞壁悬浮液处理对DON清除率高达62.85%;培养10h后不同菌体成分对DON的清除率在57%以上,其中不同浓度发酵上清液、菌体裂解液以及细胞壁悬浮液处理间存在显著差异。除细胞壁处理组外,其他各组均可将DON降解为分子质量较小的物质。结果表明,纳豆芽孢杆菌Bacillus natto 16-1各细胞成分对DON均有清除作用,且这种作用在一定程度与细胞成分的浓度及作用时间有关;各细胞成分对DON的清除机制主要是生物降解作用,细胞壁可能是通过生物吸附作用来清除DON。
This research was to investigate the efficacy and mechanisms of removal of deoxynivalenol(DON)in wheat flour by Bacillus natto 16-1 by ELISA and high performance liquid chromatography/tandem mass spectrometry(HPLC-MS).The results show that,after 5 hours of culture,the DON removal rates were above 53% in different concentrations of bacterial suspension,fermentation supernatant,bacterial lysis liquid,crude enzyme liquid and cell wall suspension.And there were significant differences among different concentrations of bacterial suspension treatments,and high concentration of cell wall suspension treatment of DON removal rate was up to 62.85%.After 10 hours of culture,the DON removal rate of different bacterial components was above 57%,and there were significant differences among different concentrations of fermentation supernatant,bacterial lysis liquid and cell wall suspension treatment.Except for the cell wall treatment group,all other groups can degrade DON as a small molecule mass substance.The cell components of Bacillus natto16-1 have a scavenging effect on DON,and this effect is somehow related to the concentration of the cell components and the treatment time.The mechanism of DON removal by each cell component is mainly biodegradation,and the cell wall may removal of DON by biosorption.
作者
张琛
许慧卿
崔桂友
丁香丽
胡舰
李波
于小番
ZHANG Chen;XU Hui-qing;CUI Gui-you;DING Xiang-li;HU Jian;LI Bo;YU Xiao-fan(College of Tourism and Culinary science Food science and Engineering,Yangzhou University,Yangzhou,Jiangsu 225127,China)
出处
《食品与机械》
北大核心
2019年第3期8-14,共7页
Food and Machinery
基金
国家自然科学基金(编号:31701634)
关键词
纳豆芽孢杆菌
呕吐毒素
脱毒
Bacillus natto
deoxynivalenol (DON)
removal
