摘要
从台州市剑门港海区的海底淤泥中采集样品分离得到4株具抗菌活性的微生物,其中一株具有较强的抗菌活性。对该菌作了形态学、生理生化、分子生物学、(G+C)mol%含量等研究,确定该菌株属于芽孢杆菌属,命名为Bacillus amyloliquefaciens MBRC1。采用BLAST Clustal W(V1.83)及Mega(V 5.1)等软件对其进行了系统发育树的分析,确立了该菌株在系统发育上的地位。采用不同的培养基对该菌株进行发酵培养,利用牛津杯法对该菌胞外的抑菌活性物质的抑菌效果进行了研究,结果显示:Bacillus amyloliquefaciens MBRC1胞外具有较强的抑菌活性物质,且对金黄色葡萄球菌(S. aureus)、枯草杆菌(B. subtilis)和大肠杆菌(E. coli)均有较好的抑菌效果。
Four bacterial strains with antibacteria activity are isolated from Jianmen harbour seafloor mud in Taizhou of Zhejiang Province,China. Thus,further studying on one of the stronger antibacteria activity strain is undertaken. This strain belongs to the genus Bacillus amyloliquefaciens,named as Bacillus amyloliquefaciens MBRC1 through molecular biology,(G+C) mol% content,colony and morphological identification. The separation position also has been identified by the analysis on phylogenetic tree with such softwares:BLAST Clustal W(V 1.83) and Mega(V 5.1).Fermented Bacillus amyloliquefaciens MBRC1 with different media and then investigates the effect of antimicrobial in extra using the method of oxford cup. The results show that Bacillus amyloliquefaciens MBRC1 could generate better antimicrobial substances and has good antimicrobial effect on S. Aureus、B. Subtilis and E. coli.
作者
陈建军
郝之奎
廖祥儒
吴翰桂
CHEN Jianjun;HAO Zhikui;LIAO Xiangru;WU Hangui(Institute of Applied Biotechnology,Taizhou Vocational & Technical College,Taizhou 318000,China;Key Laboratory of Industrial Biotechnology,Ministry of Education ,Jiangnan University,Wuxi 214122,China)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2019年第2期126-132,共7页
Journal of Food Science and Biotechnology
基金
台州市海洋生物资源开发与利用科技创新团队课题(MBR2012011)
台州市市级创新团队自主设计科技计划项目(1403ky07)
