摘要
从堆肥中分离到一株对植物病原菌尖孢镰刀菌(Fusarium oxysporum)具有强烈抗菌活性并具有较广抗菌谱的细菌Q-12菌株。通过形态观察、生理生化实验1、6S rDNA同源性序列分析以及部分特异性基因序列分析,鉴定该菌为解淀粉芽孢杆菌。该菌的最适培养基组成为:葡萄糖5g/L,NH4Cl 1g/L,牛肉膏0.8g/L,氯化镁5g/L。最适培养温度为33℃,最适培养pH为6.0,最适培养时间为40h。
Plant disease can cause serious crop losses, and chemical control of disease is costly both to the environment and to the farmer. Some microorganism can produce the substance which has the preventing and exterminating functions to plant pathogens. These substances are valid to plant pathogens with only lower concentration, in addition the substances do not remain in soil and crops without being decomposed. If composization is performed with the microorganism, or the microorganism is mixed into compost, the functional compost having preventing and exterminating action will be made out and that can be more useful to environmental preservation. In order to screen antifungal bacteria for use in biological control, 200 compost samples were taken from different regions in China, over 10 bacterium with clear antifungal activity were isolated from composts, among them, strain Q-12 exhibited the highest antifungal activity which was strongly inhibits the growth of many plant pathogenic fungi such as Fusarium oxysporum and Rhizoctonia solan. According to the characteristics of morphology, physiology and biochemistry tests （API 50 CHB/E system） and the comparison of 16S rDNA sequence, the strain Q-12 was similar to B. subtilis and B. amyloliquefaciens. Some specific genes yyaR, yyaO and tetB, which have previously been shown to be effective for resolving these closely related tara of the B. subtilis group, were analysed to clarify further the classification of Q-12, and two pairs of primers YyaR_ F/TetB. R and YyaO_ F/TetB_R were designed. From the analysis of fingerprints obtained with 1 g/L NH4Cl, 0.8 g/L beef extract, 5 g/L MgCl2, pH 6.0） at 33℃ for 40 h. A number of Bacillus strains have proven safe over many years as a nonpathogenic species and are consumed in ton quantities in several human food preparations. Strains of Bacillus have several advantages over other biocontrol bacteria in that they are often soil isolates capable of sporulation, easy to cultivate and store, and some strains have been shown to increase yields of various crops.
出处
《微生物学报》
CAS
CSCD
北大核心
2006年第1期7-12,共6页
Acta Microbiologica Sinica
基金
辽宁省自然科学基金项目(2040949)~~
关键词
解淀粉芽孢杆菌
枯草芽孢杆菌
鉴定
抑菌活性
Bacillus amyloliquefaciens
Bacillus subtilis
Identification
Antifungal activity
