摘要
目的建立糖尿病性脑内微小病变大鼠模型,进一步进行病理学分析。方法健康雄性1月龄Wistar大鼠12只,采用随机数字表法分为正常对照组(n=6)和模型组(n=6),参照赵宝珍改良的2型糖尿病模型建立方法先建立糖尿病大鼠模型,采用反复低血糖法诱导大鼠脑内微小病变的发生,饲养至6月龄进行大鼠头颅磁共振(MR)检查,筛选脑内微小病变个数≥6个、且无梗死病灶或定位体征的大鼠,定义为造模成功。造模成功后对大鼠脑组织进行病理学分析,并计数镜下低氧诱导因子-1α(HIF-1α)阳性细胞数目。结果与正常对照组相比,模型组血糖水平以及每10 g血红蛋白吸光度明显升高(t=-11.141、-38.392, P均<0.01);脑内微小病变数目增多(t=-6.104,P<0.01)。模型组脑内微小病变的病理改变主要为神经元数目减少,神经胶质细胞增生并肿胀,部分有微出血,并且HIF-1α阳性表达细胞数多于正常对照组(t=-663.45,P<0.01)。结论在糖尿病大鼠模型基础上通过反复低血糖法诱导,可建立糖尿病性脑内微小病变大鼠模型,用于探究糖尿病性脑内微小病变的发病机制及治疗靶点。
Objective To establish the rat model of intracerebral microlesions associated with diabetes, which was used for further pathological analysis. Methods A total of twelve healthy male Wistar rats were randomly divided into normal control group(n=6)and model group(n=6)according to random number table method. Initially, the diabetic rat model was established referring to the improved Zhao Baozhen′s method. Then the intracerebral microlesions was induced by recurrent hypoglycemia, and the cranial magnetic resonance(MR) examination was performed in rats until the age of 6 months.The rats showed more than six microlesions and no infarctions in the brain were defined as successful models. Pathological analysis of brain tissue from rats was conducted and the number of hypoxia inducible factor-1α(HIF-1α) positive cells were counted under the microscope. Results Compared with normal control group, blood glucose and absorbance of hemoglobin per 10 g in model group were significantly higher(t=-11.141,-38.392, all P<0.01). Moreover, the microlesions in model group were more than those in normal control group(t=-6.104, P<0.01). The pathological changes of microlesions in model group included decreasing number of neurons, the proliferation and swelling of glial cells, as well as some microhemorrhage. The HIF-1α positive cells in model group were more than those in normal control group (t=-663.45, P<0.01). Conclusion The rat model of diabetic intracerebral microlesions can be established by using the method of recurrent hypoglycemia, this model can be used to explore the pathogenesis of diabetic microlesions in the brain and potential therapeutic targets.
作者
张玉倩
刘笑迎
贾岩辉
王群
陈婡
王长德
曲红
Zhang Yuqian;Liu Xiaoying;Jia Yanhui;Wang Qun;Chen Lai;Wang Changde;Qu Hong(Department of Neurology, Binzhou Central Hospital, Shandong Province, Binzhou 251700, China;Department of Neurology, Shanghai Integrated TCM Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200082, China)
出处
《国际内分泌代谢杂志》
2019年第2期83-86,共4页
International Journal of Endocrinology and Metabolism
基金
上海市科学技术委员会中医引导类课题(16401931600)
上海市虹口区卫生和计划生育委员会重点课题(虹卫1702-05)
山东省中医药管理局中医药科技发展计划项目(2017-499).
关键词
糖尿病
脑内微小病变
大鼠
Diabetes mellitus
Intracerebral microlesion
Rat
