摘要
【目的】研究稻纵卷叶螟Cnaphalocrocismedialis对水稻、玉米和人工饲料的取食代谢效应以及取食后幼虫生长发育、物质积累和中肠消化酶活性变化。【方法】在实验室条件下以水稻叶片、玉米叶片及人工饲料饲养稻纵卷叶螟,观察生长发育状况,取特定龄期幼虫测定消化代谢、营养物质积累及中肠消化酶活性。【结果】取食水稻的稻纵卷叶螟蛹重最高、历期最短,与玉米和人工饲料差异显著,取食人工饲料幼虫历期最长。取食不同饲料后单雌产卵量、产卵历期以及成虫寿命之间无显著差异;取食水稻的食物转化率显著高于玉米和人工饲料,取食人工饲料近似消化率最高;取食水稻和玉米的幼虫体内脂肪积累量显著高于人工饲料,取食人工饲料幼虫体内蛋白质和可溶性糖含量明显增加,显著高于水稻和玉米。人工饲料的幼虫体内总蛋白酶、淀粉酶、脂肪酶三种消化酶活性显著高于水稻和玉米。【结论】不同饲料饲养对稻纵卷叶螟生长发育及营养物质累积均有影响,而稻纵卷叶螟幼虫可能通过调节取食量及消化酶活性的方式保证对食料中营养物质摄取。
[Objectives] To study the effect of different diets on the digestive efficiency, nutritional content, larval development and digestive enzyme activity of Cnaphalocrocis medialis.[Methods] The digestive efficiency, nutritional content, developmental duration and digestive enzyme activity of C. medialis larvae reared on either an artificial diet, rice leaves or maize leaves, was measured and compared.[Results] C. medialis was able to complete its life cycle on all three diets but had different developmental durations and pupal weights on each diet. C. medialis reared on rice leaves had the shortest developmental duration and the heaviest pupae. There was, however, no significant difference in the fecundity,oviposition period or adult longevity, of C. medialis reared on different diets. C. medialis reared on rice leaves were significantly more efficient at converting digested food(ECD) than those reared on maize leaves or the artificial diet. The approximate digestibility(AD) of larvae reared on the artificial diet was 52.32%, significantly higher than that of larvae reared on rice or maize leaves. C. medialis larvae reared on the artificial diet had higher soluble protein and sugar content than that of those reared on rice or maize, but lower fat content. Protease, amylase and lipase activity in the midguts of larvae fed on the artificial diet was significantly higher than that of larvae fed on rice or maize.[Conclusion] Diet affected the development and nutritional composition of C. medialis. This species appears able to ensure adequate nutritional uptake on different diets by adjusting its feeding behavior and the activity of midgut digestive enzymes.
作者
李传明
祁建杭
韩光杰
刘琴
杨亚军
吕仲贤
徐健
LI Chuan-Ming;QI Jian-Hang;HAN Guang-Jie;LIU Qin;YANG Ya-Jun;LV Zhong-Xian;XU Jian(Jiangsu Lixiahe Institute of Agricultural Science, Yangzhou 225007, China;School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China;Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China)
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2019年第1期114-121,共8页
Chinese Journal of Applied Entomology
基金
江苏省自然科学基金(BK20181215)
江苏省农科院探索性颠覆性创新计划项目(ZX(17)2002)
扬州市重点研发计划(YZ2017092)
扬州市自然科学基金(YZ2016119)
关键词
稻纵卷叶螟
取食代谢
消化酶
Cnaphalocrocis medialis
digestion response
digestive enzyme
