摘要
为改善丝素纤维人工血管材料(SFF)的生物相容性,拓展其在小口径人工血管领域的应用,采用层层组装法(LbL)在SFF表面构建再生丝素蛋白(RSF)和壳聚糖(CHI)多层膜,表征组装前后材料的元素组成和亲疏水性以及组装膜形貌、厚度和粗糙度,并进行生物相容性评价。结果表明:通过LbL法可在SFF表面成功构建(RSFz7CHI)n层膜;组装(RSF/CHI)8的SFF在1、3、5d的细胞增殖率分别为未组装SFF的1.64、2.82和4.45倍;组装(RSF/CHI)8的SFF的溶血率约为未组装试样的1/3。生物相容性试验表明,组装(RSF/CHI)8的SFF的体外细胞和血液相容性均得到显著改善。
In order to improve the biocompatibility of silk fibroin fiber vascular materials (SFF) and expand its application as small diameter vascular grafts, this paper deposited regenerated silk fibroin (RSF) and chitosan (CHI) onto SFF using layer-by-layer (LbL) assembly method. The elementary composition, water contact angle, morphology, thickness and roughness of (RSF/CHI)tt multilayer and biocompatibility of SFF materials assembled with (RSF/CHI)tt multilayer were evaluated. The results show that the (RSF/CHI)tt multilayer structure has been successfully assembled onto the SFF materials. The 1, 3 and 5 d cell proliferation rates of (RSF/CHI)8 SFF are 1. 64, 2.82 and 4.45 times of untreated SFF. The hemolysis rate of (RSF/CHI)s SFF is 1/3 of untreated SFF. The biocompatibility of SFF with (RSF/CHI)s multilayer improves dramatically compared to the untreated samples.
作者
余劭婷
吴雨芬
于成龙
李超婧
关国平
王璐
YU Shaoting;WU Yufen;YU Chenglong;LI Chaojing;GUAN Guoping;WANG Lu(College of Textiles, Ministry of Education, Donghua University, Shanghai 201620, China;Key Laboratory of Textile Science and Technology, Ministry of Education,Donghua University, Shanghai 201620, China)
出处
《东华大学学报（自然科学版）》
CAS
北大核心
2019年第1期59-66,共8页
Journal of Donghua University(Natural Science)
基金
中央高校基本科研业务费专项资助项目(2232015A-02)
上海市教育委员会科研创新资助项目(ZX201503000017)
关键词
层层组装
丝素汗维
人工血官
细胞相客性
溶血率
layer-by-layer assembly
silk fibroin fiber
vascular graft
cytocompatibility
hemolysis rate
