摘要
采用静电纺丝技术制备了聚己内酯(PCL)与胶原/丝素(COL/SF)质量比为0∶100、10∶90、20∶80、30∶70、40∶60、50∶50的复合微纳米纤维膜,通过扫描电子显微镜、力学性能测试和接触角测试等对复合纤维膜的理化性能进行表征,并将HepG_2细胞种植在复合纤维膜上检测其细胞生物相容性。结果表明:PCL/COL/SF复合微纳米纤维膜纤维形貌良好,纤维直径和亲水性随PCL含量的增加而减小;PCL/COL/SF复合纤维膜具有较好的力学性能。PCL与COL/SF质量比为30∶70时,复合纤维膜亲水性良好,强度和柔性最佳,并且HepG_2细胞在复合纤维膜表面黏附生长良好,细胞增殖情况明显,表明PCL与COL/SF质量比为30∶70的复合纤维膜有望成为一种良好的载体,可应用于体外肝细胞培养。
The different mass ratio of PCL/COL/SF composite micro-nanofibrous membranes were prepared by electrospinning, and the mass ratio of PCL and COL/SF was 0 ： 100, 10 ： 90, 20 ： 80, 30 ： 70, 40 ： 60, 50 ： 50. The membranes were eharacterized by combined techniques of seanning eleetron mieroseopy （SEM）, mechanical properties and contaet angle measurements. HepG2 cells were seeded on the composite membrane, cell adhesion and proliferation were evaluated by SEM and CCK-8. The results showed that the composite fibrous membranes could maintain the morphology, and with the increase of PCL content, the fiber diameter decreased, and the contact angle of composite fibrous membrane enhanced. The PCL/COL/ SF composite micro-nanofibrous membranes had good mechanical property. The HepG2 cell culture results showed that hepatocyte adhesion had proliferation were best when the mass ratio of PCL and COL/SF was 30 ： 70. These results showed that electrospun PCL/COUSF fibrous membrane with mass ratio of PCL and COL/SF of 30 ： 70 had a good performance and could as a good carrier in hepatocvte culture.
出处
《合成纤维》
CAS
2017年第4期47-51,共5页
Synthetic Fiber in China
基金
青年科学基金项目(51503035)
关键词
聚己内酯
胶原
丝素
微纳米纤维
肝细胞培养
polycaprolactone, collagen, silk fibroin, micro-nanofibrous, hepatocyte culture
