摘要
目的探讨CYP11A1基因启动子甲基化改变与胎儿生长受限(FGR)和或子痫前期(PE)的相关性。方法针对正常妊娠对照26例、胎儿生长受限(FGR) 40例、子痫前期(PE) 40例及胎儿生长受限合并子痫前期(FGR-PE) 18例标本,采用焦磷酸测序和实时荧光定量PCR,检测CYP11A1基因的甲基化和mRNA表达水平。结果胎盘中CYP11A1基因在FGR、FGR-PE、PE中呈低甲基化水平,尤其在FGR-PE中CYP11A1基因甲基化水平相对更低;同时,可能受到CYP11A1基因低甲基化状态的反向调控,CYP11A1基因的mRNA表达水平显著上调。结论CYP11A1基因甲基化作为胎盘组织特异性表观遗传学分子标志物,与胎儿生长受限、子痫前期等疾病相关。
Objective To investigate the correlation between CYP11A1 gene promter methylation and fetal growth restriction (FGR) and preeclampsia (PE).Methods The bisulfite pyrosequncing assay and qRT-PCR were used to demonstrate CYP11A1 methylation and expression patterns respectively in FGR,PE and FGR-PE,as well as Control associated placentas and cord blood of fetus.Results The promoter of CYP11A1 was found to be significantly hypomethylated in FGR,PE and FGR-PE placentas.And the methylation of CYP11A1 gene was relatively lower in FGR-PE than other case.Meanwhile the increased CYP11A1 expression was inversely modulate with CYP11A1 hypomethylation FGR,PE and FGR-PE placentas.which only found in placenta but not in cord blood of fetus.Conclusions As a tissue-specific epigenetic marker,CYP11A1 gene is associated with fetal growth restriction and preeclampsia or both.
作者
孟照琰
郝胜菊
张庆华
MENG Zhao-yan;HAO Sheng-ju;ZHANG Qing-hua(Department of Obstetrics,Gansu Provincial Maternity and Child-care Hospital,Lanzhou 730050,China;Department of Medicial Genetic Center,Gansu Provincial Maternity and Child-care Hospital,Lanzhou 730050,China)
出处
《基础医学与临床》
CSCD
2019年第2期203-206,共4页
Basic and Clinical Medicine
基金
兰州市科技局科技发展指导性计划项目(2014-1-1)
