摘要
目的探讨鸟嘌呤核苷酸结合蛋白(GNA12)基因启动子各位点甲基化水平、基因表达与子痫前期之间的关系。方法选取分娩孕妇中子痫前期患者(病例组)和正常孕妇(对照组)胎盘各50例,外周血各25例,采用甲基化特异性PCR技术和real-time PCR技术分析GNA12基因启动子区Cp G岛甲基化状态和mRNA的表达。结果病例组的胎盘组织及孕母外周血的GNA12 63位点的甲基化程度均低于对照组(P=0.003,P=0.004 3);病例组mRNA表达量高于对照组(P=0.013);胎盘组织甲基化率与外周血甲基化率具有相关性(P<0.000 1)。结论 GNA12基因启动子的甲基化水平与子痫前期的发病存在相关性。通过检测孕母外周血GNA12基因,可能可预测子痫前期的发生。
Objective To investigate the relationship of methylation level and gene expression of gene promoter of guanine nucleotide binding protein （GNA12） and the preeclampsia. Methods A total of 50 patients with preedampsia （preedampsia group） and 50 normal pregnant women （control group） were selected. From each group, 50 placenta tissue samples and 25 peripheral blood samples were collected. The methylation-specific PCR and real-time fluorescence quantitative PCR were adopted to analyze the methylation and mRNA expression of CpG island of gene promoter region of GNA12. Results The degree of methylation of locus 63 of GNA12 in placenta tissue and peripheral blood of the preeclampsia group were lower than those of the control group （P=0. 003, P=0. 004 3）. The mRNA expression of the preeclampsia group was higher than that of the control group （P=0. 013）. The methylation rate of placenta tissues correlated with the methylation rate of peripheral blood （P〈0. 000 1）. Cotlelusion The methylation of gene Promoter of GNA12 correlates with the incidence of preeclampsia. The incidence of preeclampsia may be predicted by detecting gene GNA12 in peripheral blood of pregnant women.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2015年第5期698-701,共4页
Journal of Shanghai Jiao tong University:Medical Science
基金
国家自然科学基金(81401186)~~
