摘要
目的通过甲醛制备小鼠炎性疼痛模型研究炎性疼痛对机体局部组织形态结构、炎症反应以及肿瘤坏死因子-α(TNF-α)和单核细胞趋化蛋白-1(MCP-1)等炎症因子表达水平的影响。方法 64只健康雄性小鼠随机分为4组,分别给予右前肢腕部注射生理盐水40μL(NS组)、50mL/L甲醛40μL(FCOH组)、5μg/mL利多卡因0.3mL(L组)、5μg/mL利多卡因0.3mL+50mL/L甲醛40μL(FCOH+L组)处理;48h后分离局部组织,HE染色观察炎细胞浸润情况;Western blot检测TNF-α和MCP-1表达水平。结果与NS组相比,FCOH组于处理24h后出现炎症反应高峰,右前肢腕部厚度增加(1.73mmvs.4.02mm,P<0.05),体温升高(37℃vs.38.3℃,P<0.05);FCOH+L组于处理48h后出现炎症反应高峰,右前肢腕部厚度显著增加(1.68mmvs.5.10mm,P<0.05),体温升高(37℃vs.38.5℃,P<0.05)。此外,处理48h后,同FCOH组相比FCOH+L组小鼠右前肢局部组织中炎性细胞浸润程度增高,而TNF-α和MCP-1表达水平降低(P<0.05)。结论炎性疼痛参与调控局部炎症反应的进程,在修复损伤组织中发挥重要作用。炎症反应发生起始阶段阻断疼痛传递可以增加中性粒细胞浸润,减少局部组织中TNF-α、MCP-1的表达,进而影响炎症反应进程的持续时间。
Objective To investigate the effects of inflammatory pain on local tissue structure,inflammatory reaction and expression levels of TNF-αand MCP-1 in formaldehyde-induced inflammatory pain in mice.Methods Sixty-four adult male mice were randomly divided into NS group(40μL of saline injected into the wrist of right forelimb),FCOH group(50 mL/L formaldehyde of 40μL injected into the wrist of right forelimb),L group(5μg/mL lidocaine of 0.3 mL for brachial plexus anesthesia)and FCOH+L group.Some of the tissue samples were collected at 48 h after formaldehyde modeling to observe the infiltration of inflammatory cells by HE staining.The rest were used to assess the expression levels of TNF-αand MCP-1 by Western blot.Results Compared with NS group,FCOH group showed peak inflammatory response at 24 h(thickness of injection sites:1.73 mm vs.4.02 mm,temperature:37℃vs.38.3℃,P<0.05).However,FCOH+L group showed intense inflammatory responses at 48 h(thickness of injection sites:1.68 mm vs.5.10 mm,temperature:37℃vs.38.5℃,P<0.05).Furthermore,after 48 h FCOH group had a lower degree of infiltration of inflammatory cells and higher expression levels of TNF-αand MCP-1 than those in FCOH+L group(P<0.05).Conclusion Inflammatory pain plays a significant role in the healing process of injured issues by facilitating the local inflammation and affecting the duration.The expression levels of TNF-αand MCP-1 in local tissues decrease by interrupting the transmission of pain.
作者
刘改霞
孙雨瑶
陈子璐
罗醒
孙月萌
王雯竹
刘进军
LIU Gai-xia;SUN Yu-yao;CHEN Zi-lu;LUO Xing;SUN Yue-meng;WANG Wen-zhu;LIU Jin-jun(Department of Physiology and Pathophysiology,Xi'an Jiaotong University Health Science Center,Xi'an 710061,China)
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2019年第1期65-70,共6页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
国家自然科学基金资助项目(No.81771616)~~
