摘要
为建立能同时快速高效的检测水产品中多种麻醉剂的检测方法,以水产品中丁香酚、甲基丁香酚、异丁香酚、乙酸丁香酚酯、甲基异丁香酚、乙酸异丁香酚酯和三卡因7种麻醉剂为检测对象,采用硅胶固相萃取柱,HP-5MS UI毛细管色谱柱,电子轰击离子源多反应监测模式(MRM)进行了相关检测,建立了能同时检测这7种麻醉剂的三重四级杆气质联用质谱仪(GC-MS/MS)分析方法。结果表明:0～100μg/L范围内7种麻醉剂的浓度和峰面积线性关系良好;确定了GC-MS/MS方法的检出限为2. 0μg/kg,定量限为5. 0μg/kg,3个水平的加标回收率为73. 93%～119. 97%,且批内和批间精密度均符合GB/T 27404-2008要求。研究表明,本研究中建立的GC-MS/MS检测方法线性相关性良好、检出限低,精密度和准确度均符合要求,能够满足对水产品中麻醉剂残留量的检测要求。
A gas chromatography-tandem mass spectrometry(GC-MS/MS)method was developed for the determination of anesthetic residues in fishery products.Solid-phase extraction(SPE)was used to purify and concentrate the target analytes which were separated by a HP-5MS UI column and detected in multiple-reaction monitoring(MRM)mode via positive electrospray ionization(ESI+).The results showed that the calibration curves of anesthetic residues were of good linearity in the range of 0-100μg/L,with detection limit of 2.0μg/kg and the quantification limit of 5.0μg/kg in this method.The recovery was found to be 73.93%-119.97% at three spiked levels for anesthetic residues and the relative standard deviation(RSD)to be able to meet requirement of GB/T27404-2008.The developed method is characterized by the excellent purification effect,sensitivity and accuracy,and can be used for the determination of anesthetic residues in fishery products.
作者
翟纹静
朱振华
王莉莉
徐振东
ZHAI Wen-jing;ZHU Zhen-hua;WANG Li-li;XU Zhen-dong(Suzhou Entry-Exit Inspection and Quarantine Bureau,Suzhou 215104,China;Suzhou Huabo Testing Technology Company Limited.,Suzhou 215104,China)
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2018年第6期769-774,共6页
Journal of Dalian Ocean University
基金
江苏出入境检验检疫局科技计划项目(2017KJ21)
