摘要
本文建立了鱼肉中MS-222残留GC-MS检测方法.鱼肉样品经乙腈提取,氮吹浓缩,盐酸溶液引导MS-222电离,Waters Oasis MCX固相萃取柱净化后,气相色谱-四极杆质谱检测.方法检出限为2.5 μg·kg-1、定量限为5.0 μg·kg-1;0.0025～1.0 μg·mL-1范围内线性关系良好（R≥0.9996）;MS-222浓度范围在5.0～ 100.0 μg·kg-1的鱼肉加标样,日内和日间平均回收率为78.4％～91.2％,相对标准偏差为3.62％ ～9.49％.结果表明,该检测方法适用于低浓度水平鱼肉中MS-222残留检测.
A method is established for detecting MS - 222 residues in fish meat by GC - MS. The MS - 222 residues is extracted with acetonitrile, ionized in HCL solution, isolated by Waters Oasis MCX SPE column, and then detected by GC - Quadrupole Mass Spectrometer. The detection limit is 2.5μg·kg^-1 and limit of quantification is 5.0μg·kg^-1, linear correlation coefficients of calibration curve is over 0. 9996 within the concentration range of 0. 0025-1.0μg·mL^-1. For the 5.0 - 100. 0μg·kg^-1 spiked sample, within - day and day -to - day average recoveries are 78.4% -91.2%, RSDs are 3.62%-9.49%. From the results of above, this method can be used to detect the low concentration MS -222 residues in fish meat.
出处
《福建水产》
2013年第6期441-447,共7页
Journal of Fujian Fisheries
基金
福建省科技厅资助项目公益类科研院所专项(2011R1003-4)
