摘要
为研究急性低盐18胁迫对刺参Apostichopus japonicus盐度调节相关基因表达的影响,在实验室条件下,以盐度30为对照组,72 h为1个盐度波动周期,采用荧光定量的方法进行了刺参(16. 93 g±3. 08 g)不同组织不同时间段单羧酸转运蛋白家族16a13 (Monocarboxylate Transporters 13,SLC16a13)基因、单羧酸转运蛋白家族6a8 (Solute Carrier Family 6 Member 8,SLC6a8)基因、甲壳素受体蛋白(Fibrinogen C Domain-Containing Protein 1,FIBCD1)基因和AMPA型谷氨酸受体1 (Glutamate Ionotropic Receptor AMPA Type Subunit 1,Gria1)基因4个与盐度调节有关的基因差异表达分析。结果表明:SLC16a13、SLC6a8、FIBCD1、Gria1基因均在刺参不同组织中检测到且有不同程度的表达,这4个基因在刺参体腔液、肠、呼吸树中均有表达;在6、12、48 h时,刺参体腔液中SLC16a13基因的表达量均显著高于对照组(P <0. 05),在肠组织中,该基因的表达量在1. 5、3 h时与对照组无显著性差异(P> 0. 05),在其余时间点均显著高于对照组(P<0. 05),在72 h时表达量达到最高,在呼吸树组织中,该基因表达量在各时间点均显著上调(P<0. 05),在72 h表达量达到最高;试验过程中,SLC6a8基因在体腔液中的表达量显著高于其他组织(P<0. 05);总体上FIBCD1基因在体腔液中表达最高,在呼吸树中次之,在肠中最低;总体上Gria1基因在肠组织中表达最高,在体腔液中次之,在呼吸树中最低。本研究结果为刺参对环境的适应机制研究提供了数据资料。
The expression profiles of four genes related-salinity regulation were detected in intestine,coelomic fluid,and respiratory tree of sea cucumber Apostichopus japonicas with body weight of(16.93±3.08)g reared at a salinity of30(as control group)which were abruptly exposed to low salinity of18for1.3,3,6,12,24,48and72h by RT-PCR,including single carboxylic acid transporter family16a13(Monocarboxylate Transporters13,SLC16a13)gene,single carboxylic acid transporter family6a8(Solute Carrier Family6member8,SLC6a8)gene,Chitin receptor protein(Fibrinogen C Domain-Containing Protein1,FIBCD1)gene,and AMPA type glutamate receptor1(Glutamate Ionotropic Receptor AMPA Type Subunit1,Gria1)gene.The results showed that there was expression of the4genes in coelomic fluid,intestine and respiratory tree significantly tree higher SLC16a13expression level than that in the control group at6h,12h,and48h(P<0.05),except in the intestine at1.5h and3h,with the maximum at72h.In the respiratory tree,the significantly up-regulated SLC16a13expression was shown,the maximal expression at72.During the experiment,SLC16a8gene was shown to be significantly higher in coelomic fluid that in the other tissues(P<0.05).The maximal FIBCD1expression level was observed in coelomic fluid,followed by the respiratory tree,and the minimum in the intestine.There was the maximal Gria1expression in the intestine,followed by coelomic fluid and the minimal in the respiratory tree.The findings appear to be the foundation for research of adaptation mechanism of sea cucumber.
作者
蒋亚男
田燚
李晓雨
商艳鹏
常亚青
JIANG Ya-nan;TIAN Yi;LI Xiao-yu;SHANG Yan-peng;CHANG Ya-qing(Key Laboratory of Mariculture & Stock Enhancement in North China s Sea,Ministry of Agriculture and Rural Affairs,Dalian Ocean University,Dalian 116023,China)
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2018年第6期696-702,共7页
Journal of Dalian Ocean University
基金
辽宁省教育厅科研项目(L201620)
农业农村部北方海水增养殖重点实验室资助项目(2015-MSENC-KF-02)
关键词
刺参
低盐胁迫
荧光定量
基因表达
Apostichopus japonicas
low salinity stress
quantitative real-time PCR
gene expression
