摘要
为探究强壮硬毛藻降解过程中的生态效应对仿刺参生长和免疫应答的影响,开展了仿刺参室内培养试验。在自然海水(对照组)和添加冷冻死亡的强壮硬毛藻150 g(湿质量,藻处理组)的自然海水中饲养仿刺参20 d,水温(17.0±0.5)℃,在养殖的第0、5、10、15、20天,测定仿刺参体壁和肠道超氧化物歧化酶、过氧化氢酶、酸性磷酸酶、碱性磷酸酶、总抗氧化能力和丙二醛等免疫指标及水体理化指标的变化情况。试验结果显示,在强壮硬毛藻降解的第0~20天,藻处理组水体氨氮和亚硝态氮质量浓度均显著高于对照组(P<0.05),仿刺参生长速率、体壁和肠道免疫相关酶活性变化显著(P<0.05)。在强壮硬毛藻降解的第0~10天,仿刺参的酸性磷酸酶、碱性磷酸酶、超氧化物歧化酶、过氧化氢酶活性和总抗氧化能力均较对照组显著升高(P<0.05)。在第15~20天,超氧化物歧化酶和过氧化氢酶活性下降,藻处理组两者活性显著低于对照组(P<0.05)。在强壮硬毛藻降解过程中,仿刺参的比生长速率为0.18%/d,显著低于对照组(P<0.05),仿刺参体壁和肠道丙二醛含量逐渐积累升高。试验结果表明,强壮硬毛藻降解引起的生态效应降低了仿刺参比生长速率,损伤了其免疫系统。试验结果可为仿刺参健康养殖和科学管理提供基础数据。
To investigate the ecological effects of the degradation of filamentous alga Chaetomorpha valida on the growth and immune response of sea cucumbers Apostichopus japonicus,an indoor cultivation experiment imitating sea cucumber was conducted.sea cucumber were reared in natural seawater(control group)and 150 g(wet weight)of frozen death filamentous alga C.valida(alga treatment group)for 20 d at water temperature of(17.0±0.5)℃.The immune indices including activities of superoxide dismutase,catalase,acid phosphatase,alkaline phosphatase,and total antioxidant capacity and malondialdehyde contents were determined in the body wall and intestine of the sea cucumber as well as the physicochemical indices in water on the 0th,5th,10th,15th and 20th days of the culture.The resulted showed that the concentrations of ammonia nitrogen and nitrite nitrogen were significantly elevated in the alga treatment group compared to the control group(P<0.05)during the C.valida degradation process,superoxide dismutase and catalase activities and total antioxidant capacity were shown significantly higher in the body wall and intestine than that in the control group from 0 d to 10 d in degradation(P<0.05).The activities of superoxide dismutase and catalase showed a decreasing trend,and both activities were significantly lower in the algal treatment group than those in the control group(P<0.05)from 15 d to 20 d.During the degradation process of C.valida,the sea cucumber had growth rate of 0.18%/d,significantly lower than the sea cucumber in the control group did(P<0.05).The levels of malondialdehyde were found to be gradually increased in the body wall and intestines of sea cucumber.The ecological effects caused by the degradation of C.valida led to reduce the growth rate of sea cucumber and to damage their immune system.The findings provide new theoretical support and basis for the healthy breeding and scientific management of A.japonicus.
作者
王鑫
邢荣莲
张洪霞
刘楚瑶
王琳倩
刘仁煜
陈丽红
徐晓光
曹学彬
程操
苏群
WANG Xin;XING Ronglian;ZHANG Hongxia;LIU Chuyao;WANG Linqian;LIU Renyu;CHEN Lihong;XU Xiaoguang;CAO Xuebin;CHENG Cao;SU Qun(Yantai Key Laboratory of Characteristic Agricultural Bioresource Conservation&Germplasm Innovative Utilization,School of Life Sciences,Yantai University,Yantai 264005,China;Cooperation and Operations Division of the Science and Education Park Management Office,Yantai University,Yantai 264005,China;Yantai Jinghai Marine Fisheries Co.,Ltd,Yantai,Shandong,Yantai 264006,China;Anyuan Marine Breeding Co.,Ltd,Yantai,Shandong,Yantai 264006,China;Baoyuan Bio-Agri Science&Technology(Shandong)Co.,Ltd,Yantai Shandong,Yantai 264006,China)
出处
《水产科学》
北大核心
2025年第4期534-543,共10页
Fisheries Science
基金
国家自然科学基金资助项目(42376155,42076110)
山东省自然科学基金资助项目(ZR2024MC161)
烟台市科技发展计划基础项目(2023JCYJ093)
烟台大学博士启动基金资助项目(SM20B133,SM22B187).
关键词
强壮硬毛藻
仿刺参
免疫
氨氮
亚硝态氮
Chaetomorpha valida
Apostichopus japonicus
immunity
ammonia nitrogen
nitrite nitrogen
