摘要
通过MS-222对2种规格的异齿裂腹鱼进行麻醉行为特征研究,为高原鱼类的麻醉以及运输提供技术支持。试验表明:MS-222麻醉大规格(25.0±1.5) cm和小规格(14.8±2.3) cm异齿裂腹鱼时,在麻醉时期3期以内,呼吸频率增加并之间无显著性差异,在麻醉4期以后呼吸频率才开始显著下降。MS-222麻醉大规格和小规格异齿裂腹鱼的有效质量浓度分别为150—180 mg/L和150 mg/L。在此浓度范围内,大规格异齿裂腹鱼在MS-222麻醉液中, 5min之内达到4级麻醉状态, 5min之内苏醒恢复,且在麻醉液中浸浴20min后成活率为100%时的浓度;小规格异齿裂腹鱼在MS-222麻醉液中, 5min之内达到4级麻醉状态, 7min之内苏醒恢复,且在麻醉液中浸浴20min后成活率为100%时的浓度。大规格异齿裂腹鱼在180 mg/L的MS-222溶液中麻醉5min,在空气中进行暴露0—15min,复苏时间无显著性差异(P>0.05);小规格异齿裂腹鱼在150 mg/L的MS-222溶液中麻醉5min,在空气中进行暴露0—15min,复苏时间存在显著性差异(P<0.05)。
This study examined anesthetic behaviors of Schizothorax o’connori Llord in two size ranges as induced by MS-222,to acquire useful information on anesthesia of said highland fish species in out-of-water activities(surgery,labeling,measurement,etc.).Test results indicated that for both large(25.0±1.5)cm and small-sized(14.8±2.3)cm specimens,respiration rates showed no significant change in anesthetic stagesⅠtoⅢ,and only begun to significantly decline after entering stageⅣ.The effective concentrations of MS-222 were 150-180 mg/L and 150 mg/L for two sizes,respectively.The 150-180 mg/L caused large specimens to enter anesthetic stageⅣwithin 5min,and recovered within 5min;the 150 mg/L caused small specimens to enter stageⅣwithin 5min,and recovered within 7min;both doses ensured a 100%survival rate after immersion for 20min.For large specimens anesthetized at 180 mg/L for 5min and exposed to air for 0-15min,there was no significant difference in recovery times(P>0.05);for small specimens anesthetized at 150 mg/L for 5min and exposed to air for 0-15min,there were significant differences in recovery(P<0.05).
作者
刘艳超
刘海平
刘书蕴
李锡峰
宋小广
LIU Yan-Chao;LIU Hai-Ping;LIU Shu-Yun;LI Xi-Feng;SONG Xiao-Guang(Institute of Fisheries Science,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa 850002,China;Tibet Agriculture and Animal Husbandry University,Nyingchi 860000,China;Tanggu Township,Linzhou County,Lhasa 850000,China)
出处
《水生生物学报》
CAS
CSCD
北大核心
2018年第6期1214-1223,共10页
Acta Hydrobiologica Sinica
基金
Supported by the Special Finance of Tibet Autonomous Region(2017CZZX004,XZNKY-2018-C-040)
Funds for Agricultrual Science,Technology and Services of Tibet Autonomous Region Finance Department(2018SFTG01)
Key R&D and Transformation Plans Tibet Autonomous Re
关键词
MS-222
麻醉
异齿裂腹鱼
MS-222
Anesthesia
Schizothorax o’connori Llord
