摘要
目的:研究黑灵芝多糖对丙烯酰胺(acrylamide,AA)所致的小肠上皮细胞氧化损伤的保护作用。方法:构建AA诱导小肠上皮细胞(intestinal epithelial cells,IEC)-6氧化损伤型,采用噻唑蓝比色法检测黑灵芝多糖对IEC-6氧化损伤的保护作用;同时测定细胞培养液中乳酸脱氢酶(lactate dehydrogenase,LDH)的漏出量,以及测定细胞中超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)及丙二醛(malondialdelyde,MDA)水平。结果:与正常对照组相比,不同质量浓度的黑灵芝多糖对细胞增殖影响无显著性差异。与模型(AA)组相比,不同质量浓度的黑灵芝多糖溶液能够明显减轻AA对细胞的毒害作用,提高细胞生存率,降低细胞培养液中LDH的释放;降低MDA含量,提高细胞SOD和GSH-Px活性。结论:黑灵芝多糖可以通过提高细胞内抗氧化酶系的活性和抗氧化物质GSH-Px活性从而有效地减弱AA诱导的小肠上皮细胞氧化损伤。
Objective: To investigate the protective effect of Ganoderma atrum polysaccharides (GAP) against oxidative injury induced by acrylamide (AA) in intestinal epithelial cells (IEC)-6. Methods: The MTT assay was used to evaluate the proliferation of IEC-6. After cultivation, the cells and culture medium were collected for determining the activities of lactate dehydrogenase (LDH), malondialdelyde (MDA), superoxide dismutase (SOD) and reduced glutathione peroxidase (GSHPx).Results: GAP had no toxic effect on the cultured IEC-6 at the tested concentrations. Compared with the AA-treated group, GAP significantly attenuated AA-induced oxidative damage and increased cell viability by decreasing LDH activity and MDA content, and increasing SOD and GSH-Px activities in IEC-6 line. Conclusion: These results suggested that GAP showed a protective effect against AA-induced oxidative damage in IEC-6 through inhibiting lipid peroxidation, and increasing antioxidant system activity.
作者
张路路
石婷
朱梦婷
陈奕
ZHANG Lulu;SHI Ting;ZHU Mengting;CHEN Yi(State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2017年第3期170-175,共6页
Food Science
基金
国家自然科学基金地区科学基金项目(21467016)
全国优秀博士学位论文作者专项资金项目(201357)
江西省青年科学家培养对象计划项目(20142BCB23005)
关键词
黑灵芝多糖
丙烯酰胺
IEC-6
氧化损伤
Ganoderma atrum polysaccharides
acrylamide
IEC-6
oxidative damage
