摘要
目的:研究黑灵芝多糖(PSG-1)对氧化应激所致大鼠乳鼠心肌细胞损伤的影响。方法:采用过氧化氢(H2O2)作用于心肌细胞,建立氧化应激损伤模型,观察细胞搏动频率,MTT法测定细胞存活率;比色法测定细胞乳酸脱氢酶(LDH)和超氧化物歧化酶(SOD)的活性及丙二醛(MDA)的含量,流式细胞仪检测细胞内的活性氧(ROS),蛋白印迹检测细胞SOD的蛋白表达。结果:PSG-1预处理可减少MDA含量,ROS产生及LDH的漏出,增加心肌细胞搏动频率、细胞存活率和SOD活性及蛋白表达并且呈剂量依赖性。结论:PSG-1对心肌细胞氧化应激损伤具有保护作用,其保护作用可能与降低MDA、ROS的含量,增强SOD活性及蛋白的表达有关。
Objective：To investigate the protective effect and its mechanisms of polysaccharides from Ganoderma atrum（PSG-1） on oxidative stress-induced cardiomyocyte injury in neonatal rats.Methods：Primary cultures of cardiomyocytes from neonatal rats with PSG-1 pretreatment were exposed to H2O2 and beating rates of cardiomyocytes were monitored under inverted microscope.Cell viability was detected by MTT assay.The activities of lactate dehydrogenase（LDH） and superoxide dismutase（SOD）,and the content of malondialdehyde（MDA） were determined by a colorimetric method.The level of intracellular reactive oxygen species（ROS） was determined using flow cytomety.SOD expression was monitored by western blot analysis.Results：PSG-1 significantly decreased LDH activity,ROS generation and MDA content,and greatly increased beating rates of cardiomyocytes,cell viability,protein expression and SOD activity in a dose-dependent manner.Conclusion：PSG-1 protects cardiomyocytes against oxidative stress-induced injury,which is probably related to the attenuation of MDA content and ROS generation and the increase of SOD expression and activity.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第11期221-225,共5页
Food Science
基金
国家"863"计划项目(2008AA10Z325)
食品科学与技术国家重点实验室目标导向资助项目(SKLF-MB-200806)
教育部"长江学者和创新团队发展计划"项目(IRT0540)
关键词
黑灵芝多糖
心肌细胞
氧化应激
抗氧化作用
Ganoderma atrum polysaccharide
cardiomyocyte
oxidative stress
antioxidant activity
