摘要
以黑灵芝为原料,采用酶法和化学法联用,从黑灵芝中提取出可溶性膳食纤维和不可溶性膳食纤维,借助响应面设计分析,考察酶解温度、酶解时间、料液比和碱提pH、碱提温度、碱提时间分别对黑灵芝可溶性膳食纤维(SDF)、不可溶性膳食纤维(IDF)得率的影响。结果表明,最佳提取工艺条件分别为:酶解温度98℃、酶解时间103min、料液比1∶32和碱提pH10、碱提温度48℃、碱提时间41min,在此条件下黑灵芝SDF得率为1.08%,IDF得率为88.68%。
Response surface methodology was applied to optimize the extraction conditions ofsoluble dietary fiber (SDF) and insoluble dietary fiber (IDF) from Ganoderma atrum. Effects of enzymolysis temperature, reaction time and material-tiquid ratio on the yield of SDF and alkaline extraction pH,temperature and time on the yield of IDF were investigated. Results exhibited that the optimum extraction conditions were as follows. enzymolysis temperature and reaction time was 98℃ and 103min,solid-liquid ratio was 1:32,alkaline extraction pH,temperature and time was 10,48℃,41min. The yield of SDF and I DF was 1.08% and 88.68%, respectively, under optimized conditions.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第14期228-232,共5页
Science and Technology of Food Industry
基金
十二五科技支撑计划(2012BAD33B06)
国家自然科学基金重点项目(31130041)
国家自然科学基金(21265011)
关键词
黑灵芝
可溶性膳食纤维
不可溶性膳食纤维
响应面分析
Ganoderma atrum
insoluble dietary fiber
soluble dietary fiber
response surface analysis