摘要
目的 用更敏感的指标评价依立雄胺的生殖毒性。方法 将精悬液与不同浓度的依立雄胺共育1h ,2h后 ,借助计算机辅助分析系统录像分析精子活力参数的变化。结果 大鼠精子给予终浓度为0 .6 ,6和 6 0 μmol·L- 1的依立雄胺 1h后 ,精子活率(MOT)较对照组分别下降 19.0 %,18.0 %,16 .0 %;2h后 ,中高剂量组的MOT较对照组分别下降9.0 %,10 .0 %,且高剂量组的前向性降低。Beagle犬给予终浓度 0 .6 ,6和 6 0 μmol·L- 1依立雄胺 1h后MOT较对照组呈下降趋势 ,但无显著性差异 ,2h后MOT较对照组分别下降 31.0 %,2 4 .9%,2 8.3%。人精子体外给药实验中 ,给予终浓度为0 .12 ,0 .2 4和 0 .96 μmol·L- 1的依立雄胺 2h后 ,曲线运动速度、直线运动速度较对照组呈下降趋势 ,但无显著性差异 ,而高剂量组的精子头侧摆幅度、精子尾摆动性及MOT较对照组分别下降 2 8.0 %,5 .0 %,15 .0 %。结论 依立雄胺对精子具有一定的直接毒性 ,这种毒性存在种属差异 ,且不表现为剂量 反应关系。
AIM To evaluate the reproductive toxicity of epristeride with a more sensitive index. METHODS The sperm samples were treated with different concentrations of epristeride in vitro, then, computer assisted sperm analysis system was used to detect sperm motion after 1 h and 2 h incubation. RESULTS The percentage of motile sperm (MOT) of rat sperm treated with epristeride (final concentrations were 0.6, 6 and 60 μmol·L -1 , respectively) were decreased 19.0%, 18.0%, and 16.0%, respectively, after 1 h, and MOT of rat sperm at middle dose and high dose levels were decreased 9.0%, 10.0, respectively, after 2 h. While straightness of rat sperm at high dose level was decreased after 2 h. In beagle dogs sperm in vitro test, MOT of epristeride groups (final concentrations were 0.6, 6 and 60 μmol·L -1 , respectively) were lower 31.0%, 24.9%, 28.3%, respectively, than that of control after 1 h (P= 0.07), and differences were significant after 2 h (P<0.05). In human sperm test in vitro, the curvilinear velocity and velocity straight line of epristeride treatment groups (final concentrations were 0.12, 0.24 and 0.96 μmol·L -1 , respectively) were slightly changed than that of control group after 2 h, but amplitude of lateral head displacement, wobble and MOT of human sperm at high dose level were decreased 28.0%, 5.0% and 15.0%, respectively, after 2 h. CONCLLUSION Epristeride might have toxic effect on sperm in different species but there was no dose response relationship.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2002年第5期363-367,共5页
Chinese Journal of Pharmacology and Toxicology
基金
上海市科技启明星计划基金资助项目(99QB14 0 0 7)
