摘要
通过对血清、pH、接毒量和收毒时间等培养条件的优化 ,确定鸡传染性法氏囊病病毒 (IBDV)在Vero细胞上增殖的最佳培养条件为 :血清 2 %,pH 7.0 ,接毒量 5∶10 0 0 0 ,收毒时间为接毒后培养 10 8h。 2 5 0ml自制搅拌瓶和 5L搅拌式生物反应器研究结果表明 ,在 2g/L微载体的Vero细胞悬浮培养系统中 ,待细胞刚长满微载体时 ,按照确定的IBDV最佳增殖条件换液培养 ,病毒可在较长一段时间维持高的病毒滴度 ,最高滴度分别可达 8.875和8.5 8(-lgTCID50 ) ,比传统转瓶生产方法至少提高 1个毒价 ,可用于IBDV规模化生产。
In flask optimization tests it was proved that 2% serum, pH 7.0, 5∶10 000 inoculation concentration of infectious bursal disease virus (IBDV) and 108 h cultivation for IBDV harvesting after its inoculation were the optimal conditions when IBDV was propagated on Vero cells. The results in 250 ml self-made spinner bottle and 5 L stirred fermentor tests indicated that IBDV could maintain higher titers for a long time and the highest titers of IBDV in spinner bottle and fermentor were 8.875 and 8.58 (-lgTCID 50) respectively when IBDV was proliferated on Vero cells using 2 g/L microcarriers in spinner bottle and fermentor and was cultivated on the optimum conditions of flask tests after Vero cells had developed a monolayer on microcarriers,which were at least one titer higher than that in the traditional roller bottle.All these results suggest that this technology can be applicated to large scale production of IBDV.
出处
《中国农业科学》
CAS
CSCD
北大核心
2002年第9期1134-1138,共5页
Scientia Agricultura Sinica
基金
北京市科技合同资助项目 ( 95 15 0 2 30 0 )
