摘要
流加培养技术是动物细胞大规模生产中主流培养技术,被广泛应用于生物制品的生产,推动了生物技术产业的发展。流加培养过程中,需合理地向细胞持续提供所需营养物质,以满足其生长代谢和产物合成所需,控制代谢副产物的积累,缓解营养物耗竭和代谢副产物积累之间的矛盾。通过对流加培养的特性分析引出其优势,从培养过程中细胞代谢及基因调控、培养工艺的优化及流加过程的检测与控制等方面进行了阐述,最后提出了现如今流加培养技术存在的问题并进行了展望。
Nowadays,fed-batch culture becomes the predominant mode of the large-scale animal cell culture process.It is widely used in the production of biological products,and promotes the development of the modem biological industry.In the process of culture,the cells should be provided with nutrient for its metabolism and products synthesis.The by-produces of the cells should also be controlled to relief the contradiction between nutrient consumption and by-produces accumulation.The advantage of fed-batch culture is drawn forth from analyzing the characteristics.In this paper,we reviewed the cell metabolism coupled with gene regulation,the optimization of culture process and control strategies linked with on-line monitor in the fed-batch culture process.On the basis of above-mentioned survey,the existent problems and prospects for animal cell cultivation were discussed.
出处
《中国畜牧兽医》
CAS
北大核心
2016年第1期176-181,共6页
China Animal Husbandry & Veterinary Medicine
基金
国家高技术研究发展计划(863计划)
生物过程关键技术及装备开发(2012AA021201)
关键词
动物细胞流加培养技术
细胞代谢
培养基
控制策略
fed-batch animal cell culture technology
cellular metabolism
medium
control strategy
