摘要
使用国产BC-7L生物反应器对BHK-21细胞悬浮培养参数进行了初步研究。通过对培养液血清含量、pH、溶氧和搅拌转速逐一进行优化研究,最终确定了BHK-21细胞悬浮培养的最佳参数为:血清浓度为5%,p H为7.40、DO为50%,搅拌转速为100 r/min。
Suspension culture parameters of BHK-21 were studied by using domestic BC-7L biorcactor. The most suitable suspension culture parameters of BHK-21 cells were determined by adjusting the factors respec tively. The most suitable parameters is 5% serum concentration, 7.40, 50% dissolved oxygen and 100 r/rain
出处
《中国动物保健》
2015年第12期69-70,共2页
China Animal Health
