摘要
本实验在模拟体液 (SBF)以静止、等于或大于骨骼肌组织内体液正常生理流率 (2 m l/ 10 0 m l· min)的流动条件下 ,研究多孔磷酸钙陶瓷上类骨磷灰石的形成。结果表明 :SBF在生理流率 (2 m l/ 10 0 ml· m in)情况下 ,材料仅在多孔磷酸钙陶瓷孔隙内部形成类骨磷灰石 ;静态 (0 ml/ 10 0 ml· m in)情况下 ,多孔磷酸钙陶瓷材料表面有类骨磷灰石形成 ;高于生理流率 (10 ml/ 10 0 m l· min)时 ,表面和断面均无类骨磷灰石形成 ,但如果增加 SBF中的 Ca2 +、HPO42 -的浓度 ,则在孔隙内部有类骨磷灰石形成。生理流速条件下的结果与多数肌肉内植入磷酸钙陶瓷试验的结果一致 :仅在多孔材料内部成骨。这个结果表明 ,比起通常使用的静态浸泡试验 ,SBF以生理流率流动的动态体外试验能够更好地模拟类骨磷灰石生长的体内环境。动态 SBF对了解类骨磷灰石形成 。
This study aimed at investigating the influence of the flow rate of simulated body fluid (SBF) (2ml/100ml·min) of body fluid in skeletal muscle upon the formation of bone-like apatite on porous calcium phosphate ceramics. The in vitro immersion experiment in SBF flowing at normal physiological rate is referred to as dynamic SBF. The results showed that bone-like apatite could only formed in the pores of porous calcium phosphate when SBF flow at physiological rate (2ml/100ml·min) of body fluid in skeletal muscle. At the same time, bone-like apatite could form both in the pores and on the surface of the samples if the flowing physiological solution is 1.5 SBF.When the flowing speed of SBF is higher than normal physiological speed (10ml/100ml·min), no bone-like apatite could be detected both on the surface and in the pores of the materials. This result is in concordance with animal experiments. The dynamic SBF simulates the biological environment of bone-like apatite formation in body better than static SBF (SBF does not flow). This method is very useful for the research of the mechanism of bone-like apatite formation, which is the key step of bone growth on biomaterials, and can be used as an effective approach to investigate mechanism of the osteoinduction of calcium phosphate in nonosseous tissues in vivo.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
2002年第3期365-369,共5页
Journal of Biomedical Engineering
基金
国家重大基础研究项目"生物医学材料的基础科学问题研究"
国家自然科学基金资助项目 ( 973G19990 6 476 0 )
