摘要
目的 检测 17例急性白血病病例中有丝分裂关卡基因 hs MAD2的表达 .方法 采取 17例急性白血病患者骨髓或外周血 ,分离单个核细胞 ,用 RT- PCR和 Western blot的方法检测 hs MAD2基因的表达 ,并与正常外周血单个核细胞进行对照 .结果 RT- PCR分析表明 ,14例急性非淋巴细胞白血病病例中的 9例、3例急性淋巴细胞白血病中的 1例 hs MAD2表达与正常对照相比有显著下降 . Western blot证实了在 8例急性非淋巴细胞性白血病病例中有 4例表达有明显下降 ,与 RT-PCR半定量分析结果一致 .结论 hs MAD2基因是白血病的肿瘤相关基因 ,可能在白血病发病中具有重要作用 .
AIM To analyze the expression of mitosis checkpoint gene hsMAD2 in acute leukemia. METHODS Bone marrow or peripheral blood samples of 17 cases of acute leukemia were obtained and their mononuclear cells were isolated. Then, we analyzed their hsMAD2 expression by using RT PCR and Western blot. Normal control blood samples were also analyzed. RESULTS RT PCR analysis showed 9 cases out of 14 cases of acute non lymphocytic leukemia and 1 case out of 4 cases of acute lymphocytic leukemia had decreased expression of hsMAD2. Western blot analysis verified that 4 cases out of 8 cases had decreased expression of hsMAD2, which is consistent with the results of RT PCR. CONCLUSION hsMAD2 gene is a leukemia related gene and may play an important role in leukemia pathogenesis.
出处
《第四军医大学学报》
北大核心
2002年第17期1570-1573,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目 (3 990 0 175 )
