摘要
基因枪作为一种新发展起来的实现生物遗传转化的工具 ,正越来越受到人们的关注。基因枪法转化频率的高低主要由微弹制备、轰击参数选择、外植体状态这三方面决定。茶树的遗传转化工作起步较晚 ,且大多数的研究都依赖农杆菌系统完成。基于基因枪技术的不受基因型限制及其转化受体广泛的特点 。
Particle bombardment, as a newly developed device for genetic transformation, now is of increasing importance in this field. The level of transformation frequency is mainly depending on the factors including microcarrier preparation, bombardment parameters and explant status. Compared to some other crops, the researches in tea plant genetic transformation are relatively behindhand. Due to its properties of genotype independence and recipient extensiveness, particle bombardment system hasalso been applied to the domain of tea plant gene engineering for facilitating the researches.
出处
《茶叶》
2002年第3期138-141,144,共5页
Journal of Tea
关键词
基因枪
遗传转化
茶树
应用前景
particle bombardment
genetic transformation
tea plant
application
