摘要
以 2个澳大利亚啤酒大麦品种为供试材料 ,研究了基因转化和植株再生过程中Cu2 + 浓度、不同受体材料、高渗处理对基因转化频率的影响。结果表明 ,Cu2 + 浓度为 5 0 μmol·L-1时可以显著地促进胚性愈伤组织的形成及植株再生 ;预培养 9d的幼胚愈伤组织作为受体材料其愈伤组织的抗性率明显高于剥取当天和预培养 3～ 4d的幼胚 ;基因枪轰击前 6h和轰击后 18h ,用 0 5mol·L-1的甘露醇进行渗透处理 。
The tested materials were 2 beer barley cultivars,Stirling and Gairdner,from Australia.The effect of Cu^(2+) concentrations,different acceptors and mannitol treatments on callus cultures and transformation frequency of barley immature embryo was studied.The results showed that(1) 5.0μmol·L^(-1) Cu^(2+) could significantly improve the embryogenic callus development and plant regeneration.(2)the immature embryos pre-cultured for 9 days had a higher transformation frequency than that of pre-cultured 3-4 days and immature embryos.(3)0.5mol·L^(-1) mannitol treatment from 6h before to 18h after bombardment could increase the transformation frequency.
出处
《山地农业生物学报》
2004年第5期377-380,共4页
Journal of Mountain Agriculture and Biology
基金
国家转基因植物研究与产业化专项基金资助项目 (JY2 0 0 3 5 0 -B -2 0 -3 )
关键词
大麦
植株再生
基因枪转化
预培养
渗透处理
愈伤组织
barley
particle bombardment transformation
pre-culture
mannitol treatment
callus
influence line
