摘要
采用兔抗魏氏梭菌 α-毒素 Ig G致敏马红细胞建立了反向间接血凝试验 ,该诊断试剂与魏氏梭菌标准株的培养上清液发生特异的凝集反应 ;在 PB缓冲液中不自凝 ;与其他致病菌培养上清无交叉反应。对现场分离样品的检测结果表明 :该方法与其生化试验结果的符合率为 97.2 %,可用于兽医临床上魏氏梭菌病的快速诊断。
A reverse indirect hemoagglutination test was first established by using the rabbit anti-α toxin IgG sensitizing horse red blood cells.The diagnostic reagent was proved to be specific and susceptible to samples of Clostridium welchii,and no cross reaction was observed to samples of other pathogenic bateria.The detecting results from field isolating strains manifested that the conforming rate reached 97.2% between this test and biochemical reaction identification,and this reagent can be used for diagnosis of disease infected by Clostridium welchii clinically.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2002年第5期465-467,共3页
Chinese Journal of Veterinary Science
基金
贵州省科学技术基金资助项目 [( 1999) 30 2 7]
关键词
应用
反向间接血凝试验
魏氏梭菌
α-毒素
动物
肠道菌
reverse indirect hemoagglutination test
the α-toxin of Clostridium welchii
detection
